LOCUS       sequence-286317-         689 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3258
FEATURES             Location/Qualifiers
     source          1..689
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             553..594
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga accagtttgg cccctcagcc
       61 ctaatcaacc tctccaattt ctcatccata aaaccggaac cagccagcac ccctccacaa
      121 ggctccatgg ccaatagtac tgcagtggta aagataccag gcactcctgg ggcaggaggt
      181 cgtcttagcc ctgaaaacaa tcaggtattg accaagaaga aattacagga cttagtaaga
      241 gaagtggatc ctaatgagca gttggatgaa gatgtggagg agatgctgct gcagattgct
      301 gatgatttta tcgagagtgt ggtgacagca gcctgtcagc ttgcgcggca tcgcaagtct
      361 agcaccctgg aggtgaaaga tgtccagctg catttagagc gccagtggaa catgtggatc
      421 ccaggatttg gctctgaaga aatccgaccc tacaaaaaag cttgcaccac agaagctcac
      481 aaacagagaa tggcattgat ccggaaaaca accaagaaat accgaacttt cttgtacaaa
      541 gtggttgata tcggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa
      601 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
      661 gacgacaaaa ggtttccaag tccactccg
//
