LOCUS       sequence-286320-        1667 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3259
FEATURES             Location/Qualifiers
     source          1..1667
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggggaggaa aaagattcag atccagcgaa tcaccgacga gcggaaccga
       61 caggtgactt tcaccaagcg gaagtttggc ctgatgaaga aggcgtatga gctgagcgtg
      121 ctatgtgact gcgagatcgc actcatcatc ttcaaccact ccaacaagct gttccagtac
      181 gccagcaccg acatggacaa ggtgctgctc aagtacacgg agtacaatga gccacacgag
      241 agccgcacca acgccgacat catcgagacc ctgaggaaga agggcttcaa cggctgcgac
      301 agccccgagc ccgacgggga ggactcgctg gaacagagcc ccctgctgga ggacaagtac
      361 cgacgcgcca gcgaggagct cgacgggctc ttccggcgct atgggtcaac tgtcccggcc
      421 cccaactttg ccatgcctgt cacggtgccc gtgtccaatc agagctcact gcagttcagc
      481 aatcccagcg gctccctggt caccccttcc ctggtgacat catccctcac ggacccgcgg
      541 ctcctgtccc cccagcagcc agcactacag aggaacagtg tgtctcctgg cctgccccag
      601 cggccagcta gtgcgggggc catgctgggg ggtgacctga acagtgctaa cggagcctgc
      661 cccagccctg ttgggaatgg ctacgtcagt gctcgggctt cccctggcct cctccctgtg
      721 gccaatggca acagcctaaa caaggtcatc cctgccaagt ctccaccccc acctacccac
      781 agcacccagc ttggagcccc cagccgcaag cccgacctgc gagtcatcac ttcccaggca
      841 ggaaaggggt taatgcatca cttgactgag gaccatttag atctgaacaa tgcccagcgc
      901 cttggggtct cccagtctac tcattcgctc accaccccag tggtttctgt ggcaacgccg
      961 agtttactca gccagggcct ccccttctct tccatgccca ctgcctacaa cacagattac
     1021 cagttgacca gtgcagagct ctcctcctta ccagccttta gttcacctgg ggggctgtcg
     1081 ctaggcaatg tcactgcctg gcaacagcca cagcagcccc agcagccgca gcagccacag
     1141 cctccacagc agcagccacc gcagccacag cagccacagc cacagcagcc tcagcagccg
     1201 caacagccac ctcagcaaca gtcccacctg gtccctgtat ctctcagcaa cctcatcccg
     1261 ggcagccccc tgccccacgt gggtgctgcc ctcacagtca ccacccaccc ccacatcagc
     1321 atcaagtcag aaccggtgtc cccaagccgt gagcgcagcc ctgcgcctcc ccctccagct
     1381 gtgttcccag ctgcccgccc tgagcctggc gatggtctca gcagcccagc cgggggatcc
     1441 tatgagacgg gagaccggga tgacggacgg ggggacttcg ggcccacact gggcctgctg
     1501 cgcccagccc cagagcctga ggctgagggc tcagctgtga agaggatgcg gcttgatacc
     1561 tggacattaa agtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1621 tatatatctt gtggaaagga cgatctcgta tattatacat cgtacac
//
