LOCUS       sequence-286326-        1007 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3261
FEATURES             Location/Qualifiers
     source          1..1007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(839..863)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             871..912
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc cccgcgcgtt cctggtgaag
       61 aagccgtgcg tctccacgtg caagaggaac tggagcgagc tccccgacga ggagcgcggc
      121 gagatctacg tgccagtcag cctgggcttc tgcccaccac agccctaccg ggagccggaa
      181 ccctctgtgg ccgaaccccc ttcctgcccg ctggctttga acatgagcct tcgagactct
      241 agctacagca tggcccccgg gccctgtgtg gtggcccagc tgccctctga agacatgggc
      301 cacttgacag acccccagag cagagaccat ggcttcctgc gcaccaagat gaaggtgacc
      361 cttggggaca gtcccagtgg agacctgttc acctgccgtg tctgccagaa ggccttcacc
      421 taccagcgca tgctgaaccg ccacatgaag tgtcacaacg acgtcaagag gcacctctgc
      481 acgtactgcg ggaagggctt caatgacacc ttcgacctca agagacacgt ccgaactcac
      541 actggcgtgc ggccctacaa gtgcagcctg tgtgacaagg ccttcacgca gcgctgctct
      601 ctggagtctc acctcaagaa gatccatggt gtgcagcaga agtacgcgta caaggagcgg
      661 cgggccaagc tgtacgtgtg tgaggagtgc ggctgcacat ctgagagcca ggagggccac
      721 gtcctgcacc tgaaggagca ccaccctgac agcccgctgc tgcgcaagac ctccaagaag
      781 gtggccgtgg cactacagaa cactgtcact tccctgctgc agggcagccc ccacctgtgc
      841 ccaactttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc tctcctcggt
      901 ctcgattcta cgtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
      961 tatatatctt gtggaaagga cgacatcagg acgtaaaatt gataagt
//
