LOCUS       sequence-286332-        1805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3263
FEATURES             Location/Qualifiers
     source          1..1805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1637..1661)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1669..1710
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg ccaccagttt ccggacagct
       61 tcgtgctggg gattattgtc attcaaggat atatctatgg agttcacctg ggatgaatgg
      121 cagctactgg attctacaca gaagtacctg tacagagatg tgatattgga aaactatcat
      181 aacctgatat cagtggggta tcatggtacc aagcctgact taatcttcaa gttggaacaa
      241 ggagaagatc catggataat aaatgccaaa atttccaggc agagctgtcc agatggctgg
      301 gaagaatggt accagaacaa tcaagatgag cttgagagta ttgaaagaag ctatgcttgt
      361 agtgtgttgg gaagacttaa tctgagcaaa acccatgatt cttcaagaca gagactctat
      421 aacacacgtg gaaaaagttt gacacaaaac tcagctccaa gcagaagtta tttaagaaag
      481 aatcctgata agtttcatgg ttatgaagaa ccatattttc ttaagcatca aagagctcat
      541 agcatagaaa aaaactgtgt gtgtagtgaa tgtgggaaag cttttcgttg taagtcacag
      601 ctcattgtac atctcagaat tcatacagga gagagacctt atgaatgcag taaatgtgaa
      661 agagccttca gtgccaagtc aaaccttaat gctcatcaga gagttcatac aggagaaaaa
      721 ccctactcat gtagtgagtg cgagaaggtc ttctctttca ggtcacagct cattgtccat
      781 caggaaattc acacaggagg gaaaccctat ggctgcagtg aatgtgggaa agcctacagt
      841 tggaaatcac agcttctttt acaccagaga agtcacacag gagtgaaacc gtatgaatgc
      901 agcgaatgtg ggaaagcctt tagtttgaag tctccattcg ttgtacacca gagaactcat
      961 acaggagtga aaccccataa atgcagtgaa tgtgggaaag cctttaggag taagtcctat
     1021 ctccttgttc acatccgaat gcatacagga gaaaaaccct atcaatgcag tgattgtggg
     1081 aaagccttca atatgaagac acaactcatt gtacatcagg gagttcacac aggaaataat
     1141 ccttatcaat gcggcgaatg tgggaaagcc tttggtagga aggaacagct cactgcacat
     1201 ctgagagctc atgcaggaga gaagccctat ggatgcagtg aatgtgggaa ggctttcagc
     1261 agcaagtcat accttgttat acataggaga acacacaccg gagagagacc ctatgaatgt
     1321 agtttgtgtg agagagcctt ttgtggaaaa tcacagctga ttatacatca gagaactcat
     1381 tcaactgaga agccctatga atgcaatgaa tgtgaaaaag cctaccctag gaaggcatca
     1441 cttcagatac accagaaaac tcattcggga gagaaacctt ttaaatgcag tgaatgtgga
     1501 aaagccttca ctcagaagtc atctctcagt gaacatcaga gagttcacac tggagagaaa
     1561 ccatggaaat gctctgaatg tgggaaatcc ttctgttgga attcagggct tcgtatacat
     1621 cggaagactc ataaatgccc aactttcttg tacaaagtgg ttgatatcgg taagcctatc
     1681 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
     1741 atttcgattt cttggcttta tatatcttgt ggaaaggacg aaaccagtgt gtgtgtcatc
     1801 aaaca
//
