LOCUS       sequence-286338-        1973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3265
FEATURES             Location/Qualifiers
     source          1..1973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      38..47
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1805..1829)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1837..1878
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggcggcgga agaggaggct
       61 gcggcgggag gtaaagtgtt gagagaggag aaccagtgca ttgctcctgt ggtttccagc
      121 cgcgtgagtc cagggacaag accaacagct atggggtctt tcagctcaca catgacagag
      181 tttccacgaa aacgcaaagg aagtgattca gacccatccc aagaagctca tagccaaact
      241 gaaaagcgga ggagagataa aatgaataac ctgattgaag aactgtctgc aatgatccct
      301 cagtgcaacc ccatggcgcg taaactggac aaacttacag ttttaagaat ggctgttcaa
      361 cacttgagat ctttaaaagg cttgacaaat tcttatgtgg gaagtaatta tagaccatca
      421 tttcttcagg ataatgagct cagacattta atccttaaga ctgcagaagg cttcttattt
      481 gtggttggat gtgaaagagg aaaaattctc ttcgtttcta agtcagtctc caaaatactt
      541 aattatgatc aggctagttt gactggacaa agcttatttg acttcttaca tccaaaagat
      601 gttgccaaag taaaggaaca actttcttct tttgatattt caccaagaga aaagctaata
      661 gatgccaaaa ctggtttgca agttcacagt aatctccacg ctggaaggac acgtgtgtat
      721 tctggctcaa gacgatcttt tttctgtcgg ataaagagtt gtaaaatctc tgtcaaagaa
      781 gagcatggat gcttacccaa ctcaaagaag aaagagcaca gaaaattcta tactatccat
      841 tgcactggtt acttgagaag ctggcctcca aatattgttg gaatggaaga agaaaggaac
      901 agtaagaaag acaacagtaa ttttacctgc cttgtggcca ttggaagatt acagccatat
      961 attgttccac agaacagtgg agagattaat gtgaaaccaa ctgaatttat aacccggttt
     1021 gcagtgaatg gaaaatttgt ctatgtagat caaagggcaa cagcgatttt aggatatctg
     1081 cctcaggaac ttttgggaac ttcttgttat gaatattttc atcaagatga ccacaataat
     1141 ttgactgaca agcacaaagc agttctacag agtaaggaga aaatacttac agattcctac
     1201 aaattcagag caaaagatgg ctcttttgta actttaaaaa gccaatggtt tagtttcaca
     1261 aatccttgga caaaagaact ggaatatatt gtatctgtca acactttagt tttgggacat
     1321 agtgagcctg gagaagcatc atttttacct tgtagctctc aatcatcaga agaatcctct
     1381 agacagtcct gtatgagtgt acctggaatg tctactggaa cagtacttgg tgctggtagt
     1441 attggaacag atattgcaaa tgaaattctg gatttacaga ggttacagtc ttcttcatac
     1501 cttgatgatt cgagtccaac aggtttaatg aaagatactc atactgtaaa ctgcaggagt
     1561 atgtcaaata aggagttgtt tccaccaagt ccttctgaaa tgggggagct agaggctacc
     1621 aggcaaaacc agagtactgt tgctgtccac agccatgagc cactcctcag tgatggtgca
     1681 cagttggatt tcgatgccct atgtgacaat gatgacacag ccatggctgc atttatgaat
     1741 tacttagaag cagagggggg cctgggagac cctggggact tcagtgacat ccagtggacc
     1801 ctcttgccaa ctttcttgta caaagtggtt gatatcggta agcctatccc taaccctctc
     1861 ctcggtctcg attctacgta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1921 tggctttata tatcttgtgg aaaggacgat ccggcccaca cacagcccct cta
//
