LOCUS       sequence-286344-        1208 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3267
FEATURES             Location/Qualifiers
     source          1..1208
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1040..1064)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1072..1113
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga agccacctcc caggcggcga
       61 gcggccccgg cgcgctatct gggcgaggtg accggtcccg cgacctggag cgctcgcgag
      121 aagcggcagc tagtgcgact cctgcaggcg cggcagggcc agccggagcc ggacgccacc
      181 gagctggccc gggagctgcg gggccggagc gaggctgaga tccgggtctt cctccagcag
      241 ctcaagggcc gcgtagcccg ggaggccatt cagaaagtgc atccgggtgg ccttcaggga
      301 ccaaggcgcc gggaggcaca gcccccagcc cccatagagg tctggacgga tctggctgag
      361 aagataacag ggccactgga agaagccctg gcagtggctt tctcgcaggt gctcaccatc
      421 gcggccacgg aaccggtcac cctcctgcac tccaagcccc ccaagcccac gcaggcccgt
      481 ggaaagcctt tgctcctgag cgcccctgga ggacaggaag accccgcccc tgaaatacct
      541 agctctgccc ctgctgcacc tagctccgca cccaggactc ctgaccctgc ccctgagaaa
      601 ccttctgagt cgtcggctgg tccctccact gaagaagact ttgctgtgga ctttgagaag
      661 atctacaagt acttgtcctc tgtctcccga agtggccgca gccccgagct ctcagcagct
      721 gagtccgctg tggtcctcga cctgctcatg tcacttccag aggagctgcc actcctgccc
      781 tgcacagccc tggttgagca tatgacggag acgtacctac gcctgacagc cccccagccc
      841 attcccgctg gagggagcct ggggcctgca gcagaagggg atggggctgg ctccaaggca
      901 ccagaggaga cccccccagc caccgagaag gccgagcaca gcgaactgaa atcgccttgg
      961 caagcagctg ggatctgtcc cctgaacccg ttcctggtgc ccctggagct tctgggtcgg
     1021 gcagccaccc ctgccaggtg cccaactttc ttgtacaaag tggttgatat cggtaagcct
     1081 atccctaacc ctctcctcgg tctcgattct acgtagtaat gaactagtcc gtaacttgaa
     1141 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaaactg tacgaacgtc
     1201 tatccggt
//
