LOCUS       sequence-286347-        1823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3268
FEATURES             Location/Qualifiers
     source          1..1823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1655..1679)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1687..1728
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cggccttgtt cctgtctgcc
       61 ccaccccagg ccgaggtgac cttcgaggac gtggctgtgt acctctcccg ggaggaatgg
      121 ggccgcctgg gccctgctca gaggggcctc tacagggacg tgatgctgga gacctacggg
      181 aacctagtct cactgggagt aggacctgca ggccccaagc ctggagtgat ctcgcagttg
      241 gagcgagggg atgagccctg ggtcctggat gttcagggca cctctgggaa agagcacctg
      301 agagtcaaca gcccagctct tgggaccaga actgagtaca aggagttgac ttcacaggag
      361 acatttggtg aggaagatcc ccagggatct gagccagtag aagcctgtga ccacatcagt
      421 aagtcagagg ggagcctgga aaagctagtg gagcagagag gccccagggc agtcacactg
      481 accaacgggg agagcagcag ggagtctggg ggaaacctca ggttgctgtc aagacctgtt
      541 cctgatcaga gacctcacaa atgtgatata tgtgagcaaa gttttgaaca gagatcatat
      601 ctcaacaacc ataagcgtgt acacaggtca aaaaaaacaa atacagttcg taactctggg
      661 gaaatcttca gtgcaaactt agttgttaaa gaagatcaga aaattcctac tgggaaaaaa
      721 ttgcattatt gcagttactg tgggaaaaca ttcaggtaca gtgccaacct tgtcaagcat
      781 cagcggcttc acactgaaga gaagccctac aaatgtgatg agtgtgggaa agccttcagc
      841 cagagctgcg agttcatcaa tcaccgaagg atgcactcag gagagattcc ctaccggtgt
      901 gacgagtgtg ggaagacatt cacccggagg cccaacctca tgaagcacca gaggattcac
      961 actggggaga aaccctacaa gtgtggggag tgtgggaagc actttagcgc ctactcttcc
     1021 ctgatttatc accagagaat ccacaccgga gagaaaccct ataaatgtaa tgactgcggg
     1081 aaagccttca gtgatggctc aatccttatc cgacatcgtc ggactcacac cggagagaag
     1141 ccatttgagt gcaaggaatg tggcaaaggc tttacacaaa gttctaacct tatccaacat
     1201 cagagaattc acactggaga gaaaccctat aaatgtaatg aatgtgagaa agctttcatt
     1261 caaaaaacca aactcgtgga acatcagaga agccacactg gagagaagcc ctatgaatgc
     1321 aatgactgtg gcaaagtttt cagccaaagc acacacctca tccagcacca gagaatccac
     1381 acaggagaga agccctacaa gtgcagcgag tgtgggaagg ccttccacaa cagttccaga
     1441 ctcatccacc accagaggct gcaccacgga gagaaaccct acagatgcag cgattgcaag
     1501 aaagccttca gccagagcac gtacttgatt cagcaccgga ggatccacac cggggagaag
     1561 ccctacaagt gcagcgagtg tgggaaggcc ttccggcaca gttccaacat gtgtcagcat
     1621 cagcggattc acctccggga ggacttctcc atgtacccaa ctttcttgta caaagtggtt
     1681 gatatcggta agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgaact
     1741 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgat
     1801 aggtattaga atagcctcgg att
//
