LOCUS       sequence-286350-        2447 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3269
FEATURES             Location/Qualifiers
     source          1..2447
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2279..2303)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2311..2352
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg aaataccaaa tccccctacc
       61 tccaaatgta tcacttactg gaaaagaaaa gtgaaatctg aatacatgcg acttcgacaa
      121 cttaaacggc ttcaggcaaa tatgggtgca aaggctttgt atgtggcaaa ttttgcaaag
      181 gttcaagaaa aaacccagat cctcaatgaa gaatggaaga agcttcgtgt ccaacctgtt
      241 cagtcaatga agcctgtgag tggacaccct tttctcaaaa agtgtaccat agagagcatt
      301 ttcccgggat ttgcaagcca acatatgtta atgaggtcac tgaacacagt tgcattggtt
      361 cccatcatgt attcctggtc ccctctccaa cagaacttta tggtagaaga tgagacggtt
      421 ttgtgcaata ttccctacat gggagatgaa gtgaaagaag aagatgagac ttttattgag
      481 gagctgatca ataactatga tgggaaagtc catggtgaag aagagatgat ccctggatcc
      541 gttctgatta gtgatgctgt ttttctggag ttggtcgatg ccctgaatca gtactcagat
      601 gaggaggagg aagggcacaa tgacacctca gatggaaagc aggatgacag caaagaagat
      661 ctgccagtaa caagaaagag aaagcgacat gctattgaag gcaacaaaaa gagttccaag
      721 aaacagttcc caaatgacat gatcttcagt gcaattgcct caatgttccc tgagaatggt
      781 gtcccagatg acatgaagga gaggtatcga gaactaacag agatgtcaga ccccaatgca
      841 cttccccctc agtgcacacc caacatcgat ggccccaatg ccaagtctgt gcagcgggag
      901 caatctctgc actccttcca cacacttttt tgccggcgct gctttaaata cgactgcttc
      961 cttcaccctt ttcatgccac ccctaatgta tataaacgca agaataaaga aatcaagatt
     1021 gaaccagaac catgtggcac agactgcttc cttttgctgg aaggagcaaa ggagtatgcc
     1081 atgctccaca acccccgctc caagtgctct ggtcgtcgcc ggagaaggca ccacatagtc
     1141 agtgcttcct gctccaatgc ctcagcctct gctgtggctg agactaaaga aggagacagt
     1201 gacagggaca caggcaatga ctgggcctcc agttcttcag aggctaactc tcgctgtcag
     1261 actcccacaa aacagaaggc tagtccagcc ccacctcaac tctgcgtagt ggaagcaccc
     1321 tcggagcctg tggaatggac tggggctgaa gaatctcttt ttcgagtctt ccatggcacc
     1381 tacttcaaca acttctgttc aatagccagg cttctgggga ccaagacgtg caagcaggtc
     1441 tttcagtttg cagtcaaaga atcacttatc ctgaagctgc caacagatga gctcatgaac
     1501 ccctcacaga agaagaaaag aaagcacaga ttgtgggctg cacactgcag gaagattcag
     1561 ctgaagaaag ataactcttc cacacaagtg tacaactacc aaccctgcga ccacccagac
     1621 cgcccctgtg acagcacctg cccctgcatc atgactcaga atttctgtga gaagttctgc
     1681 cagtgcaacc cagactgtca gaatcgtttc cctggctgtc gctgtaagac ccagtgcaat
     1741 accaagcaat gtccttgcta tctggcagtg cgagaatgtg accctgacct gtgtctcacc
     1801 tgtggggcct cagagcactg ggactgcaag gtggtttcct gtaaaaactg cagcatccag
     1861 cgtggactta agaagcacct gctgctggcc ccctctgatg tggccggatg gggcaccttc
     1921 ataaaggagt ctgtgcagaa gaacgaattc atttctgaat actgtggtga gctcatctct
     1981 caggatgagg ctgatcgacg cggaaaggtc tatgacaaat acatgtccag cttcctcttc
     2041 aacctcaata atgattttgt agtggatgct actcggaaag gaaacaaaat tcgatttgca
     2101 aatcattcag tgaatcccaa ctgttatgcc aaagtggtca tggtgaatgg agaccatcgg
     2161 attgggatct ttgccaagag ggcaattcaa gctggcgaag agctcttctt tgattacagg
     2221 tacagccaag ctgatgctct caagtacgtg gggatcgaga gggagaccga cgtcctttac
     2281 ccaactttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc tctcctcggt
     2341 ctcgattcta cgtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     2401 tatatatctt gtggaaagga cgaccaagat cctggatcat ttgatta
//
