LOCUS       sequence-286353-         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3270
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(557..581)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             589..630
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgcataaatt ttcaaattca
       61 aatagacaca agataagaca tactgaaaat aaacatttca gatgtaaaga atgtgacaaa
      121 tcactttgca tgctttcacg cctaactcaa cataaaaaaa ttcatactag agagaatttc
      181 tacaaatgtg aagagtgtgg aaaaaccttt aactggtcca caaacctttc taaacctaag
      241 aaaattcata ctggagaaaa accctacaaa tgtgaagtat gtggaaaagc ctttcaccaa
      301 tcctcaatcc ttactaaaca taagataatt cgtactggag aaaaacccta taaatgtgca
      361 cactgtggca aagcctttaa acagtcctca caccttacta gacataagat aattcatact
      421 gaagagaaac cctacaaatg tgaacaatgt ggcaaggtct ttaagcagtc cccaaccctt
      481 actaaacatc agataattta tactggagag gaaccataca aatgtgagga atgtggcaaa
      541 gcttttaacc tatctttgcc aactttcttg tacaaagtgg ttgatatcgg taagcctatc
      601 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
      661 atttcgattt cttggcttta tatatcttgt ggaaaggacg atttatcaaa ggaatcccca
      721 atttt
//
