LOCUS       sequence-286356-        1862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3271
FEATURES             Location/Qualifiers
     source          1..1862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1726..1767
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgtttgggat tcaggagaat
       61 attccgcgcg gggggacgac catgaaggag gagccgctgg gcagcggcat gaacccggtg
      121 cgctcgtgga tgcacacggc gggcgtggtg gacgccaaca cggccgccca gagcggcgtg
      181 gggctggcgc gggcgcactt cgagaagcag ccgccttcca acctccggaa atccaatttc
      241 ttccacttcg tgctggcgct ctacgatagg caggggcagc cggtggagat tgaaaggacc
      301 gcttttgtgg actttgtgga gaaagagaaa gagccaaaca acgagaaaac caacaacggc
      361 atccactata aactccagtt attgtacagc aacggagtca gaacagagca agatctgtat
      421 gttcgcctca tagattcaat gaccaaacag gccatcgtct acgagggcca ggacaagaac
      481 ccggagatgt gccgtgtgct gctgacccac gagatcatgt gcagccggtg ctgtgacaag
      541 aaaagttgtg gcaatagaaa cgaaacgccc tcagaccctg taatcattga cagattcttt
      601 ctaaagtttt tcctcaagtg caatcagaac tgtttgaaga atgcaggcaa ccctcgagat
      661 atgcggagat tccaggttgt tgtatcgaca acagtcaacg tggacggcca cgtgctggcc
      721 gtgtcagaca acatgtttgt gcacaacaat tccaaacacg ggaggcgggc ccgccgccta
      781 gacccgtcag aagccactcc gtgcatcaag gccatcagtc ccagtgaagg ctggaccacg
      841 gggggtgcca ccgtcatcat aattggcgac aacttctttg acgggctgca agttgtattc
      901 ggaactatgt tggtgtggag cgagctgata actccccatg ccatccgagt ccagaccccg
      961 ccgaggcaca ttcctggcgt cgtcgaagtg accctctcct acaaatccaa gcagttctgc
     1021 aaaggtgctc ctgggcgctt tgtctacacc gcccttaatg aaccaaccat agattacggc
     1081 tttcagaggt tgcagaaagt gatcccaaga catccgggtg atcccgaaag gttacccaag
     1141 gaggtgttac tgaagcgggc ggcggacctg gtggaagcct tatacggaat gcctcacaac
     1201 aaccaggaga tcatcttgaa gcgagcggcg gacatcgccg aggcgctgta cagcgttccc
     1261 cgcaatcaca accagatccc caccctgggc aacaaccctg cacacacggg catgatgggc
     1321 gtcaactcct tcagcagcca gctagccgtc aacgtgtcag agacgtcaca agccaacgac
     1381 caagtcggct acagtcgcaa tacaagcagc gtgtccccgc gaggctacgt ccccagcagt
     1441 actccccagc agtccaatta caacacagtc agcactagca tgaatggata tggaagtggc
     1501 gccatggcca gtctaggggt ccctggctcg cctggatttc ttaatggctc ctccgctaac
     1561 tctccctacg gcatgaaaca gaagagcgcc ttcgcgcccg tggtccggcc ccaagcctct
     1621 cctcctcctt cctgcaccag cgccaacggg aatggactgc aagctatgtc tgggctggta
     1681 gtcccgccaa tgttgccaac tctcttgtac aaagtggttg atatcggtaa gcctatccct
     1741 aaccctctcc tcggtctcga ttctacgtag taatgaacta gtccgtaact tgaaagtatt
     1801 tcgatttctt ggctttatat atcttgtgga aaggacgatg gaccggggca cacatatatt
     1861 gc
//
