LOCUS       sequence-286359-        2609 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3272
FEATURES             Location/Qualifiers
     source          1..2609
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2441..2465)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2473..2514
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc atcacaggat gaatgaaatg
       61 aacctgagtc cagtggggat ggagcagctg acttcatcct ctgtgagcaa tgccttgcca
      121 gtctcaggaa gtcacctggg attggctgcc tcacccactc acagtgccat ccctgcccca
      181 ggcctcccag tggcaattcc aaacctgggt ccctccctga gctctctgcc ttctgctctg
      241 tctttaatgc taccaatggg tattggggat cgaggggtga tgtgtgggtt acctgaaaga
      301 aactacaccc tacctccacc accttaccct cacctggaga gcagttattt cagaaccatt
      361 ctacctggca ttttatctta tttagctgac agaccacctc cacagtacat ccaccctaac
      421 tctataaatg ttgatggtaa tacagcatta tctatcacca ataacccttc agcactagat
      481 ccctatcagt ccaatggaaa tgttggatta gaaccaggca ttgtttcaat agactctcgc
      541 tctgtgaaca cacatggtgc ccaaagtctt catcccagtg atggccatga ggtggccttg
      601 gacacagcaa tcactatgga gaacgtttct agggttacca gcccaatttc gacagatgga
      661 atggcagagg agcttacgat ggacggtgtt gcaggcgagc attcccaaat cccaaatggc
      721 tccagaagtc atgaacctct gtctgtggat tctgtgagca acaaccttgc agcagacgct
      781 gtaggacatg gtggtgtgat acccatgcat gggaatggcc tggagctccc tgtggtcatg
      841 gagacagacc acattgcaag tcgggtcaat ggcatgtctg acagtgccct cagtgactcc
      901 attcacactg tggccatgag caccaactct gtaagcgtgg cactctctac ctcacacaac
      961 cttgcctccc tagaatctgt ttccctccat gaagttggcc tcagcctaga acctgtggct
     1021 gtctcctcca tcacccagga ggttgctatg gggacaggtc atgtagatgt atcttcagac
     1081 agtctttctt ttgtatcacc ttcactgcaa atggaagact ccaattcaaa caaggagaac
     1141 atggcaacct tgtttacaat ttggtgtact ctgtgtgacc gcgcctatcc ctcggactgt
     1201 cccgaacatg gaccagtgac ttttgttcct gacactccaa tagagagcag agcaaggctt
     1261 tctctcccaa agcagcttgt tctccgtcag tcaattgtgg gagcagaagt tggtgtatgg
     1321 actggagaaa ccattcctgt gcggacttgc tttggacctc taattggcca gcagagtcac
     1381 tccatggaag tagcagaatg gacagacaag gcagttaacc atatctggaa gatataccac
     1441 aatggtgtcc tagaattctg catcattaca actgatgaaa atgaatgtaa ttggatgatg
     1501 tttgtgcgca aagccaggaa ccgggaagag cagaatttgg tggcttatcc tcatgatgga
     1561 aaaatctttt tctgcacctc acaagatatc cctcctgaaa atgaactgct tttttattat
     1621 agccgagatt atgctcaaca gattggtgtt cctgaacacc cagatgtgca tctctgtaac
     1681 tgtggcaagg agtgcaattc ttacacagag ttcaaagccc atctgaccag ccacatccat
     1741 aaccatcttc ctacccaggg acatagcggc agccatgggc caagtcacag caaagaaagg
     1801 aagtggaagt gctcaatgtg cccccaagct tttatctctc cttccaaact tcatgtccac
     1861 tttatgggtc acatgggtat gaagccccac aagtgtgatt tctgtagcaa ggcttttagt
     1921 gatcccagca acctgcggac ccacctcaag atacatacag gtcagaagaa ctacaggtgt
     1981 accttgtgtg acaagtcttt cacccagaag gctcacctgg agtcccacat ggttatccac
     2041 actggggaga agaatcttaa gtgtgattac tgtgacaagt tgtttatgcg gaggcaggac
     2101 ctcaagcagc acgtgctcat ccacactcaa gaacgccaga tcaagtgtcc caagtgtgat
     2161 aagctgttct tgagaacaaa tcacttaaag aagcatctca attctcatga aggaaaacgg
     2221 gattatgtct gtgaaaaatg tacaaaggct tatctaacca aataccatct cacccgccac
     2281 ctgaaaacct gcaaagggcc cacctccagt tcgtcagcac cagaggagga agaagaggat
     2341 gactcagaag aggaagatct agcagactct gtggggacag aagactgtag gattaacagt
     2401 gctgtgtatt cagcggatga gtctctttct gcacataaat acccaacttt cttgtacaaa
     2461 gtggttgata tcggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa
     2521 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     2581 gacgaagaga ttgcctagcg atctagtac
//
