LOCUS       sequence-286362-        2093 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3273
FEATURES             Location/Qualifiers
     source          1..2093
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1925..1949)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1957..1998
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgaaaggcag taatagaaat
       61 aaggatcatt cagcagaagg agaaggggtt ggaaaacgac caaaacgaaa gtgtcttcag
      121 tggcatccat tgctagcaaa gaaacttctt gatttttcag aagaggaaga agaggaagac
      181 gaagaggagg atattgataa ggttcaactt cttggggccg atggcctaga gcaagatgtt
      241 ggtgaaactg aagatgatga atcaccagag cagcgagccc ggagaccaat gaatgcattt
      301 cttttatttt gcaaacgcca tcgctctctt gtacgtcagg aacaccccag gcttgataac
      361 cgaggtgcta ccaagatact agctgattgg tgggctgttc ttgatccaaa ggaaaagcag
      421 aaatacacag acatggccaa ggagtataag gatgcattta tgaaagcaaa tcctggctac
      481 aaatggtgtc ctaccacaaa caagcctgtg aaatccccaa cacccactgt caatccacga
      541 aagaaacttt gggccttccc atctgactct tcaagagact tgccaagccc caagaaagca
      601 aagactgaag aaatgcctca gcttaacttt ggaatggctg atcctactca aatgggaggc
      661 ctgagtatgc tgctgttagc tggagaacat gctcttggca caccagaggt atcctctggc
      721 acatgcaggc ctgatgtttc agaatctcct gaattacgtc agaagtcacc attgtttcag
      781 tttgccgaga tatcttcaag tacgtcccac tctgatgctt ctacaaagca gtgtcaaaca
      841 tctgccttgt ttcagtttgc agagatttct tcaaacactt cgcagttggg tggtgctgag
      901 cctgtaaaac gctgtggaaa gtctgcactc tttcaactgg cagaggaaaa cgaagctcag
      961 gaaaaggaaa ctcctctgat catgaagggt gttggaatga agaaagctgg acatttagtc
     1021 agagtgggac cagtgggagc aagaagttca agaagacaaa gccaaaagaa gactgtctcc
     1081 ttggctccgc aaagctggat gaagaatttg aaaaaaaatt caacagcctc cctcaatata
     1141 gtcctgttac atttgaccgg aaatgtgtac ctgtcccaag aaaaaagaag aagactggaa
     1201 atgtgtcctc agaaccgact aaaaccagca aaggtccttt ccagtctcag aaaaagaact
     1261 tattccacaa aattgtcagc aaatataagc acaaaaagga gaagcccaat gttccggaaa
     1321 aaggaagtgg ggataaatgg tcaaacaagc aactcttctt ggatgccatt caccctacag
     1381 aagccatatt ttcagaagac agaaacacca tggagcctgt tcataaggtt aaaaatatcc
     1441 catccatttt caacactcca gagccaacaa caacgcaaga acctttggtg ggcagccaaa
     1501 agagaaaagc aaggaaaacc aagattacac accttgtcag gacagcagat ggccgggtat
     1561 caccagcagg aggtactttg gatgacaaac caaaggaaca actgcagagg agtctcccta
     1621 aagcaactga gacagactgc aatgacaaat gctcacacaa caccgaggtc ggggagacgc
     1681 ggagcagtac tccagaaatg cctgccgtgt ctgcgttctt tagcctcgct gcgctggctg
     1741 aagtggcagc catggaaaat gtgcacagag gtcagaggtc aactccgctc acccatgatg
     1801 gacagccaaa agaaatgccg caggctcctg tacttatttc ctgcgctgac cagtgaagcg
     1861 ccctttcatt gtaaaacatt gtgctttacc tactacccta gccttgtctt taccgaggga
     1921 tgcttgccaa ctttcttgta caaagtggtt gatatcggta agcctatccc taaccctctc
     1981 ctcggtctcg attctacgta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     2041 tggctttata tatcttgtgg aaaggacgag aactgatcat agagggaggc tga
//
