LOCUS       sequence-286365-         527 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3274
FEATURES             Location/Qualifiers
     source          1..527
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(359..383)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             391..432
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga gtgagtcgag ctcgaagtcc
       61 agccagccct tggcctccaa gcaggaaaag gacggcactg agaagcgggg ccggggcagg
      121 ccgcgcaagc agcctccggt gagtcccggg acagcgctgg tagggagtca gaaggagccc
      181 agcgaagtgc caacacctaa gagacctcgg ggccgaccaa agggaagcaa aaacaagggt
      241 gctgccaaga cccggaaaac caccacaact ccaggaagga aaccaagggg cagacccaaa
      301 aaactggaga aggaggaaga ggagggcatc tcgcaggagt cctcggagga ggagcagtgc
      361 ccaactttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc tctcctcggt
      421 ctcgattcta cgtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
      481 tatatatctt gtggaaagga cgagacaacg tctggaagtt ccatggt
//
