LOCUS       sequence-286368-         494 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 494)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3275
FEATURES             Location/Qualifiers
     source          1..494
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(326..350)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             358..399
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga gtgagtcgag ctcgaagtcc
       61 agccagccct tggcctccaa gcaggaaaag gacggcactg agaagcgggg ccggggcagg
      121 ccgcgcaagc agcctccgaa ggagcccagc gaagtgccaa cacctaagag acctcggggc
      181 cgaccaaagg gaagcaaaaa caagggtgct gccaagaccc ggaaaaccac cacaactcca
      241 ggaaggaaac caaggggcag acccaaaaaa ctggagaagg aggaagagga gggcatctcg
      301 caggagtcct cggaggagga gcagtgccca actttcttgt acaaagtggt tgatatcggt
      361 aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgaac tagtccgtaa
      421 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga tttataaacc
      481 atcactacac gcga
//
