LOCUS       sequence-286377-         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3278
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(800..824)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             832..873
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg atcccgcggc cagcagctgc
       61 atgaggagcc tccagccccc agcccctgtc tggggctgcc ttcgaaaccc ccactcggaa
      121 ggcaatgggg cctcagggct accccactac ccgcccaccc cgttctcctt ccaccagaaa
      181 ccagacttcc tggcgacagc gacggcagcg taccctgact tctcagcctc ctgcctggca
      241 gccaccccac acagcctgcc ccaggaggag cacatcttca ctgagcagca ccccgctttc
      301 ccacagtccc ccaactggca cttccctgtc tcagacgccc ggcgcaggcc caactcaggc
      361 ccggcagggg gttccaagga aatggggacc agcagcctgg gcctggtgga caccacagga
      421 ggcccaggcg atgactacgg ggtgcttggg agcactgcca atgagacaga gaagaaatca
      481 tccaggcgga gaaaggagag ttcagacaac caggagaaca gagggaagcc ggagggcagc
      541 agcaaagccc gcaaggagag gacggccttc accaaggagc agctgcgaga gctggaggca
      601 gagtttgccc atcataacta cctgactcgg ctccgcagat atgagattgc ggtaaacctg
      661 gacctctctg agcgccaggt caaagtgtgg ttccagaacc gaaggatgaa gtggaagcgt
      721 gtgaagggag gtcagcccat ctcccccaat gggcaggacc ctgaggatgg ggactccaca
      781 gcctctccaa gttcagagtg cccaactttc ttgtacaaag tggttgatat cggtaagcct
      841 atccctaacc ctctcctcgg tctcgattct acgtagtaat gaactagtcc gtaacttgaa
      901 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaactaca aaattcgacg
      961 ttacggca
//
