LOCUS       sequence-28638-e         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Lawson ND, Scheer N, Pham VN, Kim CH, Chitnis AB, Campos-Ortega JA, 
            Weinstein BM
  TITLE     Notch signaling is required for arterial-venous differentiation 
            during embryonic vascular development.
  JOURNAL   Development. 2001 Oct . 128(19):3675-83.
  PUBMED    11585794
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2001 Oct . 128(19):3675-83."
COMMENT     Sequence Label: pCSGreen
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        44..62
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     44..61
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      104..113
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(110..112,113..115,116..628)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(155..176)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(416..435)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 attagcagag ctggttngtg aaccgtcaga tccgctaggc ttgatttagg tgacactata
       61 gaatacaagc tacttgttct ttttgcagct agcgctaccg gtcgccacca tggtgagcaa
      121 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
      181 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
      241 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
      301 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
      361 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
      421 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
      481 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
      541 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
      601 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
      661 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcan
      721 ccagtccgcc ctgagcaaaa acccaacgag a
//
