LOCUS       sequence-286380-         674 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3279
FEATURES             Location/Qualifiers
     source          1..674
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(506..530)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             538..579
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga tggtgctgga caaggaggac
       61 ggcgtgccga tgctctccgt ccagcccaaa gggaagcaga agggctgtgc gggctgtaac
      121 cgcaagatca aggaccgcta tctgctgaag gcattggaca agtactggca cgaagactgc
      181 ctcaagtgtg cctgctgtga ctgccgcctg ggcgaggtgg gctccaccct ctacaccaag
      241 gccaacctca tcctgtgccg acgcgactac ctgaggctct ttggcaccac agggaactgt
      301 gctgcttgca gcaagctgat cccagccttc gagatggtga tgcgggcccg ggacaacgtg
      361 tatcacctcg actgcttcgc ctgccagctc tgcaaccaga gattttgtgt gggagacaaa
      421 ttcttcctga agaacaacat gatcttgtgt cagatggact atgaggaagg gcagctcaat
      481 ggcacctttg aatcccaagt tcagtaccca actttcttgt acaaagtggt tgatatcggt
      541 aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgaac tagtccgtaa
      601 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga tttatagtac
      661 tatcgaggga actc
//
