LOCUS       sequence-286383-        1487 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3280
FEATURES             Location/Qualifiers
     source          1..1487
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1319..1343)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1351..1392
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg ctcccaagaa acgcccagaa
       61 acccagaaga cctccgagat tgtattacgc cccaggaaca agaggagcag gagtcccctg
      121 gagctggagc ccgaggccaa gaagctctgt gcgaagggct ccggtcctag cagaagatgt
      181 gactcagact gcctctgggt ggggctggct ggcccacaga tcctgccacc atgccgcagc
      241 atcgtcagga ccctccacca gcataagctg ggcagagctt cctggccatc tgtccagcag
      301 gggctccagc agtccttttt gcacactctg gattcttacc ggatattaca aaaggctgcc
      361 ccctttgaca ggagggctac atccttggcg tggcacccaa ctcaccccag caccgtggct
      421 gtgggttcca aagggggaga tatcatgctc tggaattttg gcatcaagga caaacccacc
      481 ttcatcaaag ggattggagc tggagggagc atcactgggc tgaagtttaa ccctctcaat
      541 accaaccagt tttacgcctc ctcaatggag ggaacaacta ggctgcaaga ctttaaaggc
      601 aacattctac gagtttttgc cagctcagac accatcaaca tctggttttg tagcctggat
      661 gtgtctgcta gtagccgaat ggtggtcaca ggagacaacg tggggaacgt gatcctgctg
      721 aacatggacg gcaaagagct ttggaatctc agaatgcaca aaaagaaagt gacgcatgtg
      781 gccctgaacc catgctgtga ttggttcctg gccacagcct ccgtagatca aacagtgaaa
      841 atttgggacc tgcgccaggt tagagggaaa gccagcttcc tctactcgct gccgcacagg
      901 catcctgtca acgcagcttg tttcagtccc gatggagccc ggctcctgac cacggaccag
      961 aagagcgaga tccgagttta ctctgcttcc cagtgggact gccccctggg cctgatcccg
     1021 caccctcacc gtcacttcca gcacctcaca cctatcaagg cagcctggca tcctcgctac
     1081 aacctcattg ttgtgggccg atacccagat cctaatttca aaagttgtac cccttatgaa
     1141 ttgaggacga tcgacgtgtt cgatggaaac tcagggaaga tgatgtgtca gctctatgac
     1201 ccagaatctt ctggcatcag ttcgcttaat gaattcaatc ccatggggga cacgctggcc
     1261 tctgcaatgg gttaccacat tctcatctgg agccaggagg aagccaggac acggaagtgc
     1321 ccaactttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc tctcctcggt
     1381 ctcgattcta cgtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1441 tatatatctt gtggaaagga cgaactgtca cgagttctaa caagcag
//
