LOCUS       sequence-286386-        2165 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3281
FEATURES             Location/Qualifiers
     source          1..2165
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1997..2021)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2029..2070
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cattcaggga tgtggctgtg
       61 gatttcaccc aggatgagtg gaggctgctg agccctgctc aaaggactct gtacagagag
      121 gtgatgctgg agaactacag caacctggtc tcactgggaa tttcattttc taaaccagaa
      181 ctcatcaccc agctggagca agggaaagag acctggagag aggaaaaaaa atgttcaccg
      241 gcaacctgtc cagatccaga gccagagctc tacctcgatc ctttctgccc tccgggtttc
      301 tccagtcaga aattccccat gcagcatgtg ctgtgtaatc atcccccctg gatcttcaca
      361 tgcttgtgtg cagaaggtaa catccagcct ggggatccag gcccagggga ccaggagaag
      421 cagcaacaag cctctgaggg gagaccctgg agtgatcaag cagaaggtcc tgagggagaa
      481 ggtgccatgc ctttgtttgg aagaaccaag aaaaggactc tgggagcgtt ctccaggcca
      541 ccccagaggc agccagtcag ctctcggaac ggcctcagag gggtggagtt agaagccagc
      601 ccagctcaga cagggaaccc tgaggaaaca gacaaattgt tgaagaggat agaagtctta
      661 ggatttggaa cagtcaactg tggagagtgt ggactgagct tcagcaagat gacaaacctg
      721 ctcagtcacc agcggataca ctcaggggag aagccctacg tgtgtggggt atgtgagaag
      781 ggcttcagcc taaagaagag cctcgccaga caccagaagg cacactcggg ggagaagcca
      841 attgtgtgca gggagtgtgg acgaggcttt aaccggaagt caacgctaat catacacgaa
      901 cggacacact ccggtgagaa accttacatg tgcagtgagt gtgggcgagg cttcagccag
      961 aagtcaaacc tcatcataca ccagaggaca cactcagggg aaaagcctta tgtgtgccgg
     1021 gaatgtggca aaggcttcag ccagaagtca gctgtcgtga gacaccagag gacacacttg
     1081 gaggagaaga ccatcgtgtg cagtgactgt ggcctgggct tcagcgacag gtcaaacctc
     1141 atctcccacc agaggacgca ctctggggag aagccctacg cctgcaagga gtgtgggcga
     1201 tgcttcaggc agaggaccac ccttgtcaac caccagagga cacactcaaa ggagaagccc
     1261 tatgtgtgcg gggtgtgtgg gcacagcttc agccagaatt caaccctcat ctctcacagg
     1321 cggacacaca ctggggagaa gccgtatgtt tgtggggtgt gtgggcgagg ctttagtctc
     1381 aagtcacacc tcaacagaca ccagaacata cactcaggag agaagcccat tgtgtgcaag
     1441 gactgtggcc ggggcttcag ccagcaatcc aacctcatca gacaccagag gacgcactca
     1501 ggcgagaagc ccatggtgtg tggggagtgc gggcgaggct tcagccagaa gtcaaacctt
     1561 gttgcacacc agaggacgca ctcaggggag aggccgtatg tgtgccgaga gtgtgggcga
     1621 ggctttagcc accaggccgg tctcatcagg cacaagcgga agcactcgag ggagaagccc
     1681 tacatgtgca ggcagtgtgg actgggcttt ggcaataagt cagctctaat cacacacaag
     1741 cgggctcact cggaagagaa gccttgtgtg tgcagagagt gtggccaagg ctttctccaa
     1801 aagtcacacc tcaccttaca tcaaatgaca catacggggg agaagccata tgtgtgcaag
     1861 acgtgtgggc ggggcttcag cctcaagtct cacctcagca gacacaggaa gaccacgtct
     1921 gtccaccaca gactgccagt gcagcccgac cctgagccgt gtgcagggca accttcggat
     1981 tccttatact ctctctgccc aactttcttg tacaaagtgg ttgatatcgg taagcctatc
     2041 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
     2101 atttcgattt cttggcttta tatatcttgt ggaaaggacg agcaaacatt tgtgacatta
     2161 cgaaa
//
