LOCUS       sequence-286389-        2303 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3282
FEATURES             Location/Qualifiers
     source          1..2303
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2135..2159)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2167..2208
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg atttatctgc tcttcgcgtt
       61 gaagaagtac aaaatgtcat taatgctatg cagaaaatct tagagtgtcc catctgtctg
      121 gagttgatca aggaacctgt ctccacaaag tgtgaccaca tattttgcaa attttgcatg
      181 ctgaaacttc tcaaccagaa gaaagggcct tcacagtgtc ctttatgtaa gaatgatata
      241 accaaaagga gcctacaaga aagtacgaga tttagtcaac ttgttgaaga gctattgaaa
      301 atcatttgtg cttttcagct tgacacaggt ttggagtatg caaacagcta taattttgca
      361 aaaaaggaaa ataactctcc tgaacatcta aaagatgaag tttctatcat ccaaagtatg
      421 ggctacagaa accgtgccaa aagacttcta cagagtgaac ccgaaaatcc ttccttgcag
      481 gaaaccagtc tcagtgtcca actctctaac cttggaactg tgagaactct gaggacaaag
      541 cagcggatac aacctcaaaa gacgtctgtc tacattgaat tgggatctga ttcttctgaa
      601 gataccgtta ataaggcaac ttattgcagt gtgggagatc aagaattgtt acaaatcacc
      661 cctcaaggaa ccagggatga aatcagtttg gattctgcaa aaaaggctgc ttgtgaattt
      721 tctgagacgg atgtaacaaa tactgaacat catcaaccca gtaataatga tttgaacacc
      781 actgagaagc gtgcagctga gaggcatcca gaaaagtatc agggtgaagc agcatctggg
      841 tgtgagagtg aaacaagcgt ctctgaagac tgctcagggc tatcctctca gagtgacatt
      901 ttaaccactc agcagaggga taccatgcaa cataacctga taaagctcca gcaggaaatg
      961 gctgaactag aagctgtgtt agaacagcat gggagccagc cttctaacag ctacccttcc
     1021 atcataagtg actcttctgc ccttgaggac ctgcgaaatc cagaacaaag cacatcagaa
     1081 aaagtattaa cttcacagaa aagtagtgaa taccctataa gccagaatcc agaaggcctt
     1141 tctgctgaca agtttgaggt gtctgcagat agttctacca gtaaaaataa agaaccagga
     1201 gtggaaaggt catccccttc taaatgccca tcattagatg ataggtggta catgcacagt
     1261 tgctctggga gtcttcagaa tagaaactac ccatctcaag aggagctcat taaggttgtt
     1321 gatgtggagg agcaacagct ggaagagtct gggccacacg atttgacgga aacatcttac
     1381 ttgccaaggc aagatctaga gggaacccct tacctggaat ctggaatcag cctcttctct
     1441 gatgaccctg aatctgatcc ttctgaagac agagccccag agtcagctcg tgttggcaac
     1501 ataccatctt caacctctgc attgaaagtt ccccaattga aagttgcaga atctgcccag
     1561 agtccagctg ctgctcatac tactgatact gctgggtata atgcaatgga agaaagtgtg
     1621 agcagggaga agccagaatt gacagcttca acagaaaggg tcaacaaaag aatgtccatg
     1681 gtggtgtctg gcctgacccc agaagaattt atgctcgtgt acaagtttgc cagaaaacac
     1741 cacatcactt taactaatct aattactgaa gagactactc atgttgttat gaaaacagat
     1801 gctgagtttg tgtgtgaacg gacactgaaa tattttctag gaattgcggg aggaaaatgg
     1861 gtagttagct atttctgggt gacccagtct attaaagaaa gaaaaatgct gaatgagcat
     1921 gattttgaag tcagaggaga tgtggtcaat ggaagaaacc accaaggtcc aaagcgagca
     1981 agagaatccc aggacagaaa gatcttcagg gggctagaaa tctgttgcta tgggcccttc
     2041 accaacatgc ccacagggtg tccacccaat tgtggttgtg cagccagatg cctggacaga
     2101 ggacaatggc ttccatgcaa ttgggcagat gtgtgcccaa ctttcttgta caaagtggtt
     2161 gatatcggta agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgaact
     2221 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgat
     2281 gtgcggccgc caccacgtgc gca
//
