LOCUS       sequence-286392-        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3283
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(848..872)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             880..921
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga gctcttattt tgtaaactca
       61 ttttgcggtc gctatccaaa tggcccggac taccagttgc ataattatgg agatcatagt
      121 tccgtgagcg agcaattcag ggactcggcg agcatgcact ccggcaggta cggctacggc
      181 tacaatggca tggatctcag cgtcggccgc tcgggctccg gccactttgg ctccggagag
      241 cgcgcccgca gctacgctgc cagcgccagc gcggcgcccg ccgagcccag gtacagccag
      301 ccggccacgt ccacgcactc tcctcagccc gatccgctgc cctgctccgc cgtggccccc
      361 tcgcccggca gcgacagcca ccacggcggg aaaaactccc taagcaactc cagcggcgcc
      421 tcggccgacg ccggcagcac ccacatcagc agcagagagg gggttggcac ggcgtccgga
      481 gccgaggagg acgcccctgc cagcagcgag caggcgagtg cgcagagcga gccgagcccg
      541 gcgccgcccg cccaacccca gatctacccc tggatgcgca agctgcacat aagtcatgac
      601 aacataggcg gcccggaagg caaaagggcc cggacggcct acacgcgcta ccagaccctg
      661 gagctggaga aggagttcca cttcaaccgt tacctgaccc gcagaaggag gattgaaata
      721 gcacatgctc tttgcctctc cgagagacaa attaaaatct ggttccaaaa ccggagaatg
      781 aagtggaaaa aagataataa gctgaaaagc atgagcatgg ccgcggcagg aggggccttc
      841 cgtccctgcc caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
      901 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
      961 tcttggcttt atatatcttg tggaaaggac gatcatggct gactccctag aacatg
//
