LOCUS       sequence-286395-        1028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3284
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(860..884)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             892..933
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga accttctccg agaacagagt
       61 agaacacgtc ccatttctcc aaaagagatt gaaagaatgg tgggcatcat ccatcgaaaa
      121 tttagttcca ttcagatgca gctcaaacaa agcacttgtg aagcagttat gattttaaga
      181 tcaaggttcc ttgatgccag acggaaaagg cgtaacttca gtaaacaggc cacagaaatc
      241 ttgaatgaat atttttactc acacctcagc aacccctacc ccagtgaaga agccaaagag
      301 gagctggcca agaaatgcag catcacagtg tcacagagtc ttgtaaagga tccaaaagaa
      361 agagggagca aaggttctga catccagcca actagcgtgg tatccaattg gtttggcaac
      421 aaacgaatca ggtacaagaa gaacattggc aagtttcagg aagaagccaa cctctatgct
      481 gcaaagacgg ccgtgacagc tgcacacgca gtagcagcag ctgtgcagaa caaccagacc
      541 aattcgccca ccacaccaaa ttccggttct tctggttctt ttaacctccc aaattctggg
      601 gacatgttca tgaacatgca gagtctgaat ggggattctt accaagggtc ccaagtcgga
      661 gccaatgtgc aatcacaggt ggataccctc cgtcatgtta tcaatcagac gggaggctac
      721 agtgatggcc ttggaggaaa ttcactgtac agtccacata atttaaatgc taatggaggc
      781 tggcaggacg caacaactcc atcttctgtg acttctccta cagaaggccc aggaagtgtg
      841 cactcggata cctctaacta cccaactttc ttgtacaaag tggttgatat cggtaagcct
      901 atccctaacc ctctcctcgg tctcgattct acgtagtaat gaactagtcc gtaacttgaa
      961 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaaagcg ggcgcatttg
     1021 cagtcact
//
