LOCUS       sequence-286398-        1634 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3285
FEATURES             Location/Qualifiers
     source          1..1634
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1466..1490)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1498..1539
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggacagtag ctgccacaac
       61 gcgactacca aaatgttagc gactgctcca gctcggggca acatgatgag cacgtccaaa
      121 cccttggctt tctccattga acgaatcatg gcgcgcaccc cagagcccaa ggccctgcca
      181 gtcccccact tcctgcaggg agccttaccc aagggggaac ccaagcactc tctgcatctc
      241 aactcgtcga tcccctgcat gatccccttc gtgcctgtgg cctacgacac gagccccaag
      301 gcaggagtga cgggctccga gccgcggaag gccagtctgg aggccccggc ggcgcccgcg
      361 gcggtgccct cggctcccgc attcagctgc agcgacctgc tcaactgcgc actgagtctc
      421 aagggcgacc tggcccgcga cgcgctgccg ctgcagcagt acaagctggt aaggccgcgt
      481 gtggtcaacc actcttcatt ccacgccatg ggcgccttgt gctacctgaa ccgaggtgac
      541 ggcccatgcc acccggcagc cggcgtgaac atccacccgg tggcctccta cttcctcagt
      601 tcccctttgc acccgcagcc aaaaacgtat ttagccgaaa ggaataaact ggtggtcccg
      661 gcggtggaga aatacccttc tggagtagct ttcaaagacc tgtcccaggc tcagctgcag
      721 cattacatga aagaaagcgc ccagcttctg tcggaaaaaa tcgcgttcaa aacctcggat
      781 ttcagccgag gctctcctaa tgccaagccc aaagttttca cttgcgaagt gtgtggaaag
      841 gtctttaatg cgcactataa cttaacccgt cacatgccag tgcacacagg agccagaccc
      901 ttcgtttgca aagtgtgcgg aaaaggtttc aggcaagcaa gcaccctgtg caggcacaag
      961 atcattcaca cgcaggaaaa acctcacaaa tgtaaccagt gtggcaaagc atttaataga
     1021 agttccactt taaacactca tacccgaata cacgcgggct acaaaccgtt tgtgtgtgaa
     1081 ttctgtggca aagggtttca tcaaaaaggg aattacaaaa accacaagtt gacccacagc
     1141 ggggagaagc agttcaagtg caatatctgc aacaaggctt tccaccaggt ttacaacctc
     1201 accttccaca tgcacaccca caacgacaag aagcctttca cctgccccac gtgcggcaag
     1261 ggtttctgca ggaactttga cctcaagaag catgtccgca agctgcacga cagcagcctg
     1321 gggctggccc gcacgccagc tggcgaacca ggcactgaac cgccgccccc gctaccgcag
     1381 cagccgccga tgacgctgcc tcctctgcag ccgccgctgc caaccccggg gcccctgcag
     1441 cccgggctcc accagggcca ccagttgcca actttcttgt acaaagtggt tgatatcggt
     1501 aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgaac tagtccgtaa
     1561 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga cgatgatccc
     1621 ggcttgtgcg tgag
//
