LOCUS       sequence-286401-        1421 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3286
FEATURES             Location/Qualifiers
     source          1..1421
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1253..1277)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1285..1326
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggccatggt gaccggcggc
       61 tggggcggcc ccggcggcga cacgaacggc gtggacaagg cgggcggcta cccgcgcgcg
      121 gccgaggacg actcggcctc gccccccggt gccgccagcg acgccgagcc gggcgacgag
      181 gagcggccgg ggctgcaggt ggactgcgtg gtgtgcgggg acaagtcgag cggcaagcat
      241 tacggtgtct tcacctgcga gggctgcaag agctttttca agcgaagcat ccgccgcaac
      301 ctcagctaca cctgccggtc caaccgtgac tgccagatcg accagcacca ccggaatcag
      361 tgccagtact gccgtctcaa gaagtgcttc cgggtgggca tgaggaagga ggcggtgcag
      421 cgcggccgca tcccgcactc gctgcctggt gccgtggccg cctcctcggg cagccccccg
      481 ggctcggcgc tggcggcagt ggcgagcggc ggagacctct tcccggggca gccggtgtcc
      541 gaactgatcg cgcagctgct gcgcgctgag ccctaccctg cggcggccgg acgcttcggc
      601 gcagggggcg gcgcggcggg cgcagtgctg ggcatcgaca acgtgtgcga gctggcggcg
      661 cggctgctct tcagcaccgt ggagtgggcg cgccacgcgc ccttcttccc cgagctgccg
      721 gtggccgacc aggtggcgct gctgcgcctg agctggagcg agctcttcgt gctgaacgcg
      781 gcgcaggcgg cgctgcccct gcacacggcg ccgctactgg ccgccgccgg cctccacgcc
      841 gcgcctatgg ccgccgagcg cgccgtggct ttcatggacc aggtgcgcgc cttccaggag
      901 caggtggaca agctgggccg cctgcaggtc gactcggccg agtatggctg cctcaaggcc
      961 atcgcgctct tcacgcccga cgcctgtggc ctctcagacc cggcccacgt tgagagcctg
     1021 caggagaagg cgcaggtggc cctcaccgag tatgtgcggg cgcagtaccc gtcccagccc
     1081 cagcgcttcg ggcgcctgct gctgcggctc cccgccctgc gcgcggtccc tgcctccctc
     1141 atctcccagc tgttcttcat gcgcctggtg gggaagacgc ccattgagac actgatcaga
     1201 gacatgctgc tgtcggggag taccttcaac tggccctacg gctcgggcca gttgccaact
     1261 ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct cggtctcgat
     1321 tctacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1381 tcttgtggaa aggacgatcc tgcaatttat gtttccaaac g
//
