LOCUS       sequence-28641-e         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Villefranc JA, Amigo J, Lawson ND
  TITLE     Gateway compatible vectors for analysis of gene function in the 
            zebrafish.
  JOURNAL   Dev Dyn. 2007 Nov . 236(11):3077-87.
  PUBMED    17948311
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2007 Nov . 236(11):3077-87."
COMMENT     Sequence Label: pCSDest
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    91..225
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(128..147)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     182..201
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    270..394
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(537..556)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ccatgcattg gcgccgcggc cgcgaaatta aaaaacctcc cacacctccc cctgaacctg
       61 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
      121 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
      181 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatctacgt aatacgactc
      241 actatagttc tagaggctcg agaggatcaa ccactttgta caagaaagct gaacgagaaa
      301 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
      361 aatactgtaa aacacaacat atccagtcac tatggtcgac ctgcagactg gctgtgtata
      421 agggagcctg acatttatat tccccagaac atcaggttaa tggcgttttt gatgtcattt
      481 tcgcggtggc tgagatcagc cacttcttcc ccgataacgg agaccggcac actggccata
      541 tcggtggtca tcatgcgcca gctttcatcc ccgatatgca ccaccgggta aagttcacgg
      601 gagactttat ctgacagcag acgtgcactg gccaggggga tcaccatccg tcgcccgggc
      661 gtgtcaataa tatcactcgg tacatccacn aacagacgat aacggctctc tcttttatag
      721 gtgtaaacct taactgcatt tcccag
//
