LOCUS       sequence-286413-        2387 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3290
FEATURES             Location/Qualifiers
     source          1..2387
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2219..2243)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2251..2292
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg aaggtgatgc agtcgaagcc
       61 attgtggagg agtccgaaac ttttattaaa ggaaaggaga gaaagactta ccagagacgc
      121 cgggaagggg gccaggaaga agatgcctgc cacttacccc agaaccagac ggatgggggt
      181 gaggtggtcc aggatgtcaa cagcagtgta cagatggtga tgatggaaca gctggacccc
      241 acccttcttc agatgaagac tgaagtaatg gagggcacag tggctccaga agcagaggct
      301 gctgtggacg atacccagat tataacttta caggttgtaa atatggagga acagcccata
      361 aacataggag aacttcagct tgttcaagta cctgttcctg tgactgtacc tgttgctacc
      421 acttcagtag aagaacttca gggggcttat gaaaatgaag tgtctaaaga gggccttgcg
      481 gaaagtgaac ccatgatatg ccacacccta cctttgcctg aagggtttca ggtggttaaa
      541 gtgggggcca atggagaggt ggagacacta gaacaagggg aacttccacc ccaggaagat
      601 cctagttggc aaaaagaccc agactatcag ccaccagcca aaaaaacaaa gaaaaccaaa
      661 aagagcaaac tgcgttatac agaggagggc aaagatgtag atgtgtctgt ctacgatttt
      721 gaggaagaac agcaggaggg tctgctatca gaggttaatg cagagaaagt ggttggtaat
      781 atgaagcctc caaagccaac aaaaattaaa aagaaaggtg taaagaagac attccagtgt
      841 gagctttgca gttacacgtg tccacggcgt tcaaatttgg atcgtcacat gaaaagccac
      901 actgatgaga gaccacacaa gtgccatctc tgtggcaggg cattcagaac agtcaccctc
      961 ctgaggaatc accttaacac acacacaggt actcgtcctc acaagtgccc agactgcgac
     1021 atggcctttg tgaccagtgg agaattggtt cggcatcgtc gttacaaaca cacccacgag
     1081 aagccattca agtgttccat gtgcgattac gccagtgtag aagtcagcaa attaaaacgt
     1141 cacattcgct ctcatactgg agagcgtccg tttcagtgca gtttgtgcag ttatgccagc
     1201 agggacacat acaagctgaa aaggcacatg agaacccatt caggggaaaa gccttatgaa
     1261 tgttatattt gtcatgctcg gtttacccaa agtggtacca tgaagatgca cattttacag
     1321 aagcacacag aaaatgtggc caaatttcac tgtccccact gtgacacagt catagcccga
     1381 aaaagtgatt tgggtgtcca cttgcgaaag cagcattcct atattgagca aggcaagaaa
     1441 tgccgttact gtgatgctgt gtttcatgag cgctatgccc tcatccagca tcagaagtca
     1501 cacaagaatg agaagcgctt taagtgtgac cagtgtgatt acgcttgtag acaggagagg
     1561 cacatgatca tgcacaagcg cacccacacc ggggagaagc cttacgcctg cagccactgc
     1621 gataagacct tccgccagaa gcagcttctc gacatgcact tcaagcgcta tcacgacccc
     1681 aacttcgtcc ctgcggcttt tgtctgttct aagtgtggga aaacatttac acgtcggaat
     1741 accatggcaa gacatgctga taattgtgct ggcccagatg gcgtagaggg ggaaaatgga
     1801 ggagaaacga agaagagtaa acgtggaaga aaaagaaaga tgcgctctaa gaaagaagat
     1861 tcctctgaca gtgaaaatgc tgaaccagat ctggacgaca atgaggatga ggaggagcct
     1921 gccgtagaaa ttgaacctga gccagagcct cagcctgtga ccccagcccc accacccgcc
     1981 aagaagcgga gaggacgacc ccctggcaga accaaccagc ccaaacagaa ccagccaaca
     2041 gctatcattc aggttgaaga ccagaataca ggtgcaattg agaacattat agttgaagta
     2101 aaaaaagagc cagatgctga gcccgcagag ggagaggaag aggaggccca gccagctgcc
     2161 acagatgccc ccaacggaga cctcacgccc gagatgatcc tcagcatgat ggaccggtgc
     2221 ccaactttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc tctcctcggt
     2281 ctcgattcta cgtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     2341 tatatatctt gtggaaagga cgaaccacac cgtttgggac acatctc
//
