LOCUS       sequence-286416-        1322 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3291
FEATURES             Location/Qualifiers
     source          1..1322
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1154..1178)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1186..1227
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cgaagcagta cgactcggtg
       61 gagtgccctt tttgtgatga agtttccaaa tacgagaagc tcgccaagat cggccaaggc
      121 accttcgggg aggtgttcaa ggccaggcac cgcaagaccg gccagaaggt ggctctgaag
      181 aaggtgctga tggaaaacga gaaggagggg ttccccatta cagccttgcg ggagatcaag
      241 atccttcagc ttctaaaaca cgagaatgtg gtcaacttga ttgagatttg tcgaaccaaa
      301 gcttccccct ataaccgctg caagggtagt atatacctgg tgttcgactt ctgcgagcat
      361 gaccttgctg ggctgttgag caatgttttg gtcaagttca cgctgtctga gatcaagagg
      421 gtgatgcaga tgctgcttaa cggcctctac tacatccaca gaaacaagat cctgcatagg
      481 gacatgaagg ctgctaatgt gcttatcact cgtgatgggg tcctgaagct ggcagacttt
      541 gggctggccc gggccttcag cctggccaag aacagccagc ccaaccgcta caccaaccgt
      601 gtggtgacac tctggtaccg gcccccggag ctgttgctcg gggagcggga ctacggcccc
      661 cccattgacc tgtggggtgc tgggtgcatc atggcagaga tgtggacccg cagccccatc
      721 atgcagggca acacggagca gcaccaactc gccctcatca gtcagctctg cggctccatc
      781 acccctgagg tgtggccaaa cgtggacaac tatgagctgt acgaaaagct ggagctggtc
      841 aagggccaga agcggaaggt gaaggacagg ctgaaggcct atgtgcgtga cccatacgca
      901 ctggacctca tcgacaagct gctggtgctg gaccctgccc agcgcatcga cagcgatgac
      961 gccctcaacc acgacttctt ctggtccgac cccatgccct ccgacctcaa gggcatgctc
     1021 tccacccacc tgacgtccat gttcgagtac ttggcaccac cgcgccggaa gggcagccag
     1081 atcacccagc agtccaccaa ccagagtcgc aatcccgcca ccaccaacca gacggagttt
     1141 gagcgcgtct tctgcccaac tttcttgtac aaagtggttg atatcggtaa gcctatccct
     1201 aaccctctcc tcggtctcga ttctacgtag taatgaacta gtccgtaact tgaaagtatt
     1261 tcgatttctt ggctttatat atcttgtgga aaggacgatg cattgattca catcgactac
     1321 tc
//
