LOCUS       sequence-286419-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3292
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(656..680)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             688..729
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg aacccttggc cagcaacatc
       61 caggtcctgc tgcaggcggc cgagttcctg gagcgccgtg agagagaggc cgagcatggt
      121 tatgcgtccc tgtgcccgca tcgcagtcca ggccccatcc acaggaggaa gaagcgaccc
      181 ccccaggctc ctggcgcgca ggacagcggg cggtcagtgc acaatgaact ggagaagcgc
      241 aggagggccc agttgaagcg gtgcctggag cggctgaagc agcagatgcc cctgggggcc
      301 gactgtgccc ggtacaccac gctgagcctg ctgcgccgtg ccaggatgca catccagaag
      361 ctggaggatc aggagcagcg ggcccgacag ctcaaggaga ggctgcgcag caagcagcag
      421 agcctgcagc ggcagctgga gcagctccgg gggctggcag gggcggccga gcgggagcgg
      481 ctgcgggcgg acagtctgga ctcctcaggc ctctcctctg agcgctcaga ctcagaccaa
      541 gaggagctgg aggtggatgt ggagagcctg gtgtttgggg gtgaggccga gctgctgcgg
      601 ggcttcgtcg ccggccagga gcacagctac tcgcacggcg gcggcgcctg gctatgccca
      661 actttcttgt acaaagtggt tgatatcggt aagcctatcc ctaaccctct cctcggtctc
      721 gattctacgt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
      781 atatcttgtg gaaaggacga cacgatgttc acaggcaggg tatg
//
