LOCUS       sequence-28642-e         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Villefranc JA, Amigo J, Lawson ND
  TITLE     Gateway compatible vectors for analysis of gene function in the 
            zebrafish.
  JOURNAL   Dev Dyn. 2007 Nov . 236(11):3077-87.
  PUBMED    17948311
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2007 Nov . 236(11):3077-87."
COMMENT     Sequence Label: pCSDest2
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    100..234
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(137..156)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     191..210
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    274..397
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(536..555)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 cgggtcgggc cnatgcattg gcgccgcggc cgcgaattaa aaaacctccc acacctcccc
       61 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
      121 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
      181 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatctacgta
      241 atacgactca ctatagttct agaggctcga gtgcaactat gtataataaa gttgaacgag
      301 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta
      361 cataatactg taaaacacaa catatccagt cactatggac tgcagactgg ctgtgtataa
      421 gggagcctga catttatatt ccccagaaca tcaggttaat ggcgtttttg atgtcatttt
      481 cgcggtggct gagatcagcc acttcttccc cgataacgga gaccggcaca ctggccatat
      541 cggtggtcat catgcgccag ctttcatccc cgatatgcac caccgggtaa agttcacggg
      601 agactttatc tgacagcaga cgtgcactgg ccagggggat caccatccgt cgcccgggcg
      661 tgtcaataat atcactctgt acatccacaa acagaagana ccggctctct cttttatagg
      721 tgtaaacctt aantgcattc accagccccg tggtctc
//
