LOCUS       sequence-286422-        1190 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3293
FEATURES             Location/Qualifiers
     source          1..1190
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1022..1046)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1054..1095
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgcaaagaaa tgcaatgtat
       61 ttaaagaatg tggcagagac tgcttgtaac tttcaactaa ctcaatatca aataagtcat
      121 gctaatcaga aaccctatga atgtcagata tgtggaaagc cctttagaaa gcgtgcccac
      181 cttacccaac ataatcgaat tcacactggt gggaaaccat atgaatgcaa agaatgtggg
      241 aaagtgttta tctgttgttc aacattgatt caacataaga gaactcacac tagtgagaaa
      301 ccctatgaat gtctggaatg tagaaagacg tttaggcgga gtgcacatct tattcgacat
      361 caaagaattc atactggtga gaaaccttat aaatgtaagc aatgttggaa ggcctttgct
      421 tctgtttctg atttaataga catcggaaaa ttcacactga tgagagactt tacgaatgta
      481 cagaatgtgg gaaggcattt aacaatcgct caactcttat tcagcatcag agaattcaca
      541 ctggtgagaa gtcctttgaa tgtaaggaat gtggcaaagc attcaataat tgctcaacac
      601 ttactcaaca ccagagaact catactaatg agaaatctta tgaatgtcag gaatgtaaaa
      661 aggcttttag gcaaagtgca catcttactc aacatcaaag aattcatact ggtgagaaac
      721 catatgagtg taagtaatgt gggaaggctt tcacttgtgt ttctgatttt aatagacatc
      781 agagaattca cactggtgaa aaaccctatg aatgtaaaga atgtggtaga gcttttaaca
      841 attgttcagc tcttattcaa caccagagaa ttcacactgg tgagaaggct tatgaatata
      901 agcagtgtgg gaaggttttt gtctcctgtc caacacttgt tcaacatcag agaacacata
      961 ctaatgaaaa accttatgaa tgtcagtaat gcaagaaggc cttcaagcat aatgcacatc
     1021 ttgccaactt tcttgtacaa agtggttgat atcggtaagc ctatccctaa ccctctcctc
     1081 ggtctcgatt ctacgtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1141 ctttatatat cttgtggaaa ggacgaatat cagttcttgg ggctgtgtgt
//
