LOCUS       sequence-286425-        1175 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3294
FEATURES             Location/Qualifiers
     source          1..1175
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1007..1031)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1039..1080
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg ccgctgcgcg cccagaggcc
       61 cagagtcgga gctcaccgac tccggagtcg cgatcccagg agccactgga cctggtcctg
      121 gtgcctgatg actgccggcc tggcacaccc ccgagtgacc tcatcgagat ccaggtggtg
      181 aaggtgacgg acaccacgct ggtccctgag cccccggagc caggttcttt ccactgtgcc
      241 ttgtgccctg ctgccttccg gctggtttcc gagctgctgt tccacgaaca tggccacttg
      301 gctggggccg agggaggcgg gcagggtggg gacccgagcc ggtgccacgt gtgcggccac
      361 agctgcccgg gccccgccag cctgcgcgcg cactacagct tgcacacggg ggagcggccc
      421 taccgctgcg cgctctgccc ccgcgccttc aaggccttgg cgcccctgct ccggcaccag
      481 caccggcacg gggtggagcc gggcacctct cggaggcctc cggacacagc ggccgttgca
      541 gaacagaggc ccggggtggc cccggagagg gcggaggtgg tgatggcggc ggcggcggcg
      601 ggcgcagcgg tggggaagcc ttttgcctgc aggttctgcg ccaagccctt ccgccgctcc
      661 tcagacatgc gagaccacga gcgcgtgcac actggcgagc ggccatacca ttgcggcatc
      721 tgcggcaagg gcttcaccca gtcctcggtg cttagcggcc acgcccgcat ccacactggc
      781 gagcgcccgt tccgctgcac gctctgcgac cgcaccttca acaactcctc caacttccgc
      841 aagcaccagc gcacccactt ccacgggccg gggccggggc tgggagactc tggaggccag
      901 ctgggctcgt cggcggctga ggggtcgggg agcgggtgtg gggtagggga ccctgcggag
      961 gaggggcggg gggagaccgc gaaggtgaag gtggaggccg accagtaccc aactttcttg
     1021 tacaaagtgg ttgatatcgg taagcctatc cctaaccctc tcctcggtct cgattctacg
     1081 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     1141 ggaaaggacg aacacaatgc tgctctcagg cactc
//
