LOCUS       sequence-286428-        1211 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3295
FEATURES             Location/Qualifiers
     source          1..1211
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1043..1067)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1075..1116
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg gcagcgccag cccgggtctg
       61 agcagcgtat cccccagcca cctcctgctg ccccccgaca cggtgtcgcg gacaggcttg
      121 gagaaggcgg cagcgggggc agtgggtctc gagagacggg actggagtcc cagtccaccc
      181 gccacgcccg agcagggcct gtccgccttc tacctctcct actttgacat gctgtaccct
      241 gaggacagca gctgggcagc caaggcccct ggggccagca gtcgggagga gccacctgag
      301 gagcctgagc agtgcccggt cattgacagc caagccccag cgggcagcct ggacttggtg
      361 cccggcgggc tgaccttgga ggagcactcg ctggagcagg tgcagtccat ggtggtgggc
      421 gaagtgctca aggacatcga gacggcctgc aagctgctca acatcaccgc agatcccatg
      481 gactggagcc ccagcaatgt gcagaagtgg ctcctgtgga cagagcacca ataccggctg
      541 ccccccatgg gcaaggcctt ccaggagctg gcgggcaagg agctgtgcgc catgtcggag
      601 gagcagttcc gccagcgctc gcccctgggt ggggatgtgc tgcacgccca cctggacatc
      661 tggaagtcag cggcctggat gaaagagcgg acttcacctg gggcgattca ctactgtgcc
      721 tcgaccagtg aggagagctg gaccgacagc gaggtggact catcatgctc cgggcagccc
      781 atccacctgt ggcagttcct caaggagttg ctactcaagc cccacagcta tggccgcttc
      841 attaggtggc tcaacaagga gaagggcatc ttcaaaattg aggactcagc ccaggtggcc
      901 cggctgtggg gcatccgcaa gaaccgtccc gccatgaact acgacaagct gagccgctcc
      961 atccgccagt attacaagaa gggcatcatc cggaagccag acatctccca gcgcctcgtc
     1021 taccagttcg tgcaccccat ctgcccaact ttcttgtaca aagtggttga tatcggtaag
     1081 cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag tccgtaactt
     1141 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaga tacactttat
     1201 taatgcgaat c
//
