LOCUS       sequence-286431-        2684 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3296
FEATURES             Location/Qualifiers
     source          1..2684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2516..2540)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2548..2589
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg agggcgctgg cggcgcgaac
       61 gacaagaaaa agataagttc tgaacgtcga aaagaaaagt ctcgagatgc agccagatct
      121 cggcgaagta aagaatctga agttttttat gagcttgctc atcagttgcc acttccacat
      181 aatgtgagtt cgcatcttga taaggcctct gtgatgaggc ttaccatcag ctatttgcgt
      241 gtgaggaaac ttctggatgc tggtgatttg gatattgaag atgacatgaa agcacagatg
      301 aattgctttt atttgaaagc cttggatggt tttgttatgg ttctcacaga tgatggtgac
      361 atgatttaca tttctgataa tgtgaacaaa tacatgggat taactcagtt tgaactaact
      421 ggacacagtg tgtttgattt tactcatcca tgtgaccatg aggaaatgag agaaatgctt
      481 acacacagaa atggccttgt gaaaaagggt aaagaacaaa acacacagcg aagctttttt
      541 ctcagaatga agtgtaccct aactagccga ggaagaacta tgaacataaa gtctgcaaca
      601 tggaaggtat tgcactgcac aggccacatt cacgtatatg ataccaacag taaccaacct
      661 cagtgtgggt ataagaaacc acctatgacc tgcttggtgc tgatttgtga acccattcct
      721 cacccatcaa atattgaaat tcctttagat agcaagactt tcctcagtcg acacagcctg
      781 gatatgaaat tttcttattg tgatgaaaga attaccgaat tgatgggata tgagccagaa
      841 gaacttttag gccgctcaat ttatgaatat tatcatgctt tggactctga tcatctgacc
      901 aaaactcatc atgatatgtt tactaaagga caagtcacca caggacagta caggatgctt
      961 gccaaaagag gtggatatgt ctgggttgaa actcaagcaa ctgtcatata taacaccaag
     1021 aattctcaac cacagtgcat tgtatgtgtg aattacgttg tgagtggtat tattcagcac
     1081 gacttgattt tctcccttca acaaacagaa tgtgtcctta aaccggttga atcttcagat
     1141 atgaaaatga ctcagctatt caccaaagtt gaatcagaag atacaagtag cctctttgac
     1201 aaacttaaga aggaacctga tgctttaact ttgctggccc cagccgctgg agacacaatc
     1261 atatctttag attttggcag caacgacaca gaaactgatg accagcaact tgaggaagta
     1321 ccattatata atgatgtaat gctcccctca cccaacgaaa aattacagaa tataaatttg
     1381 gcaatgtctc cattacccac cgctgaaacg ccaaagccac ttcgaagtag tgctgaccct
     1441 gcactcaatc aagaagttgc attaaaatta gaaccaaatc cagagtcact ggaactttct
     1501 tttaccatgc cccagattca ggatcagaca cctagtcctt ccgatggaag cactagacaa
     1561 agttcacctg agcctaatag tcccagtgaa tattgttttt atgtggatag tgatatggtc
     1621 aatgaattca agttggaatt ggtagaaaaa ctttttgctg aagacacaga agcaaagaac
     1681 ccattttcta ctcaggacac agatttagac ttggagatgt tagctcccta tatcccaatg
     1741 gatgatgact tccagttacg ttccttcgat cagttgtcac cattagaaag cagttccgca
     1801 agccctgaaa gcgcaagtcc tcaaagcaca gttacagtat tccagcagac tcaaatacaa
     1861 gaacctactg ctaatgccac cactaccact gccaccactg atgaattaaa aacagtgaca
     1921 aaagaccgta tggaagacat taaaatattg attgcatctc catctcctac ccacatacat
     1981 aaagaaacta ctagtgccac atcatcacca tatagagata ctcaaagtcg gacagcctca
     2041 ccaaacagag caggaaaagg agtcatagaa cagacagaaa aatctcatcc aagaagccct
     2101 aacgtgttat ctgtcgcttt gagtcaaaga actacagttc ctgaggaaga actaaatcca
     2161 aagatactag ctttgcagaa tgctcagaga aagcgaaaaa tggaacatga tggttcactt
     2221 tttcaagcag taggaattgg aacattatta cagcagccag acgatcatgc agctactaca
     2281 tcactttctt ggaaacgtgt aaaaggatgc aaatctagtg aacagaatgg aatggagcaa
     2341 aagacaatta ttttaatacc ctctgattta gcatgtagac tgctggggca atcaatggat
     2401 gaaagtggat taccacagct gaccagttat gattgtgaag ttaatgctcc tatacaaggc
     2461 agcagaaacc tactgcaggg tgaagaatta ctcagagctt tggatcaagt taactgacca
     2521 actttcttgt acaaagtggt tgatatcggt aagcctatcc ctaaccctct cctcggtctc
     2581 gattctacgt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     2641 atatcttgtg gaaaggacga cccacaatgc ggtacgtgca ggga
//
