LOCUS       sequence-286437-        2459 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3298
FEATURES             Location/Qualifiers
     source          1..2459
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2291..2315)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2323..2364
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg gccagactgg gaagaaatct
       61 gagaagggac cagtttgttg gcggaagcgt gtaaaatcag agtacatgcg actgagacag
      121 ctcaagaggt tcagacgagc tgatgaagta aagagtatgt ttagttccaa tcgtcagaaa
      181 attttggaaa gaacggaaat cttaaaccaa gaatggaaac agcgaaggat acagcctgtg
      241 cacatcctga cttctgtgag ctcattgcgc gggactaggg agtgttcggt gaccagtgac
      301 ttggattttc caacacaagt catcccatta aagactctga atgcagttgc ttcagtaccc
      361 ataatgtatt cttggtctcc cctacagcag aattttatgg tggaagatga aactgtttta
      421 cataacattc cttatatggg agatgaagtt ttagatcagg atggtacttt cattgaagaa
      481 ctaataaaaa attatgatgg gaaagtacac ggggatagag aatgtgggtt tataaatgat
      541 gaaatttttg tggagttggt gaatgccctt ggtcaatata atgatgatga cgatgatgat
      601 gatggagacg atcctgaaga aagagaagaa aagcagaaag atctggagga tcaccgagat
      661 gataaagaaa gccgcccacc tcggaaattt ccttctgata aaatttttga agccatttcc
      721 tcaatgtttc cagataaggg cacagcagaa gaactaaagg aaaaatataa agaactcacc
      781 gaacagcagc tcccaggcgc acttcctcct gaatgtaccc ccaacataga tggaccaaat
      841 gctaaatctg ttcagagaga gcaaagctta cactcctttc atacgctttt ctgtaggcga
      901 tgttttaaat atgactgctt cctacatcgt aagtgcaatt attcttttca tgcaacaccc
      961 aacacttata agcggaagaa cacagaaaca gctctagaca acaaaccttg tggaccacag
     1021 tgttaccagc atttggaggg agcaaaggag tttgctgctg ctctcaccgc tgagcggata
     1081 aagaccccac caaaacgtcc aggaggccgc agaagaggac ggcttcccaa taacagtagc
     1141 aggcccagca cccccaccat taatgtgctg gaatcaaagg atacagacag tgatagggaa
     1201 gcagggactg aaacgggggg agagaacaat gataaagaag aagaagagaa gaaagatgaa
     1261 acttcgagct cctctgaagc aaattctcgg tgtcaaacac caataaagat gaagccaaat
     1321 attgaacctc ctgagaatgt ggagtggagt ggtgctgaag cctcaatgtt tagagtcctc
     1381 attggcactt actatgacaa tttctgtgcc attgctaggt taattgggac caaaacatgt
     1441 agacaggtgt atgagtttag agtcaaagaa tctagcatca tagctccagc tcccgctgag
     1501 gatgtggata ctcctccaag gaaaaagaag aggaaacacc ggttgtgggc tgcacactgc
     1561 agaaagatac agctgaaaaa ggacggctcc tctaaccatg tttacaacta tcaaccctgt
     1621 gatcatccac ggcagccttg tgacagttcg tgcccttgtg tgatagcaca aaatttttgt
     1681 gaaaagtttt gtcaatgtag ttcagagtgt caaaaccgct ttccgggatg ccgctgcaaa
     1741 gcacagtgca acaccaagca gtgcccgtgc tacctggctg tccgagagtg tgaccctgac
     1801 ctctgtctta cttgtggagc cgctgaccat tgggacagta aaaatgtgtc ctgcaagaac
     1861 tgcagtattc agcggggctc caaaaagcat ctattgctgg caccatctga cgtggcaggc
     1921 tgggggattt ttatcaaaga tcctgtgcag aaaaatgaat tcatctcaga atactgtgga
     1981 gagattattt ctcaagatga agctgacaga agagggaaag tgtatgataa atacatgtgc
     2041 agctttctgt tcaacttgaa caatgatttt gtggtggatg caacccgcaa gggtaacaaa
     2101 attcgttttg caaatcattc ggtaaatcca aactgctatg caaaagttat gatggttaac
     2161 ggtgatcaca ggataggtat ttttgccaag agagccatcc agactggcga agagctgttt
     2221 tttgattaca gatacagcca ggctgatgcc ctgaagtatg tcggcatcga aagagaaatg
     2281 gaaatccctt gcccaacttt cttgtacaaa gtggttgata tcggtaagcc tatccctaac
     2341 cctctcctcg gtctcgattc tacgtagtaa tgaactagtc cgtaacttga aagtatttcg
     2401 atttcttggc tttatatatc ttgtggaaag gacgatcgga caaaatctca ttttcataa
//
