LOCUS       sequence-28644-e         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Villefranc JA, Amigo J, Lawson ND
  TITLE     Gateway compatible vectors for analysis of gene function in the 
            zebrafish.
  JOURNAL   Dev Dyn. 2007 Nov . 236(11):3077-87.
  PUBMED    17948311
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2007 Nov . 236(11):3077-87."
COMMENT     Sequence Label: pENTRDsRedEx2
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    74..98
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    119..175
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     complement(235..258)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(433..452)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     650..670
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
ORIGIN
        1 caatatgatt tattttgact gatagtgacc tgtttcgttg caacaaattg ataagcaatg
       61 ctttcttata atgccaactt tgtacaagaa agctgggtat cagttatcta gatccggtgg
      121 atcccgggcc cgcggtaccg tcgactgcag aattcgaagc ttgagctcga gatctcagga
      181 acaggtggtg gcggccctcg gcgcgctcgt actgctccac gatggtgtag tcctcgttgt
      241 gggaggtgat gtccagcttg gagtccacgt agtagtagcc gggcagctgc acgggcttct
      301 tggccatgta gatggacttg aactccacca ggtagtggcc gccgtccttc agcttcaggg
      361 ccttgtggat ctcgcccttc agcacgccgt cgcgggggta caggcgctcg gtggaggcct
      421 cccagcccat agtcttcttc tgcattacgg ggccgtcgga ggggaagttc acgccgatga
      481 acttcacctt gtagatgaag gagccgtcct gcagggagga gtcctgggtc acggtcacca
      541 cgccgccgtc ctcgaagttc atcacgcgct cccacttgaa gccctcgggg aaggacagct
      601 tcttgtagtc ggggatgtcg gcggggtgct tcacgtacac cttggagccg tactggaact
      661 ggggggacag gatgtcccag gcgaagggca gggggccgcc cttggtcacc ttcagtgggg
      721 ggttggggtg cc
//
