LOCUS       sequence-286440-        1616 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3299
FEATURES             Location/Qualifiers
     source          1..1616
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1448..1472)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1480..1521
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cgcaaaggta cgatgagctg
       61 ccccattacg gcgggatgga cggagtaggg gttcccgctt ccatgtacgg agaccctcac
      121 gcgccgcggc cgatcccccc ggttcaccac ctgaaccacg ggccgccgct ccacgccaca
      181 cagcactacg gcgcgcacgc cccgcacccc aatgtcatgc cggccagtat gggatccgct
      241 gtcaacgacg ccttgaagcg ggacaaggac gcgatctatg ggcacccgtt gtttcctctg
      301 ttagctctgg tctttgagaa gtgcgagctg gcgacctgca ctccccggga acctggagtg
      361 gctggcggag acgtctgctc ctccgactcc ttcaacgagg acatcgcggt cttcgccaag
      421 caggttcgcg ccgaaaagcc acttttttcc tcaaatccag agctggacaa tttgatgata
      481 caagcaatac aagtactaag gtttcatctt ttggagttag aaaaggtcca cgaactgtgc
      541 gataacttct gccaccgata cattagctgt ttgaagggga aaatgcccat cgacctcgtc
      601 attgatgaaa gagacggcag ctccaagtca gatcatgaag aactttcagg ctcctccaca
      661 aatctcgctg accataaccc ttcttcttgg cgagaccacg atgatgcaac ctcaacccac
      721 tcagcaggca ccccagggcc ctccagtggg ggccatgctt cccagagcgg agacaacagc
      781 agtgagcaag gggatggttt agacaacagt gtagcttcac ctggtacagg tgacgatgat
      841 gatccggata aggacaaaaa acgccagaag aaaagaggca ttttccccaa agtagcaaca
      901 aatatcatga gagcatggct cttccagcat ctcacacatc cgtacccttc cgaagagcag
      961 aagaaacagt tagcgcaaga cacaggactt acaattctcc aagtaaacaa ctggtttatt
     1021 aatgccagaa gaagaatagt acagcccatg attgaccagt caaatcgagc agtgagccaa
     1081 ggagcagcat atagtccaga gggtcagccc atggggagct ttgtgttgga tggtcagcaa
     1141 cacatgggga tccggcctgc aggtttgcag agcatgccag gggactacgt ttctcagggt
     1201 ggtcctatgg gaatgagtat ggcacagcca agttacactc ctccccagat gaccccacac
     1261 cctactcaat taagacatgg acccccaatg cattcatatt tgccaagcca tccccaccac
     1321 ccagccatga tgatgcacgg aggaccccct acccaccctg gaatgactat gtcagcacag
     1381 agccccacaa tgttaaattc tgtagatccc aatgttggcg gacaggttat ggacattcat
     1441 gcccaatacc caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
     1501 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
     1561 tcttggcttt atatatcttg tggaaaggac gaactgcggc tgatcagatc cccagg
//
