LOCUS       sequence-286443-        1349 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3300
FEATURES             Location/Qualifiers
     source          1..1349
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1181..1205)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1213..1254
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg acggagtagg ggttcccgct
       61 tccatgtacg gagaccctca cgcgccgcgg ccgatccccc cggttcacca cctgaaccac
      121 gggccgccgc tccacgccac acagcactac ggcgcgcacg ccccgcaccc caatgtcatg
      181 ccggccagta tgggatccgc tgtcaacgac gccttgaagc gggacaagga cgcgatctat
      241 gggcacccgt tgtttcctct gttagctctg gtctttgaga agtgcgagct ggcgacctgc
      301 actccccggg aacctggagt ggctggcgga gacgtctgct cctccgactc cttcaacgag
      361 gacatcgcgg tcttcgccaa gcaggttcgc gccgaaaagc cacttttttc ctcaaatcca
      421 gagctggaca atttgatgat acaagcaata caagtactaa ggtttcatct tttggagtta
      481 gaaaaggtcc acgaactgtg cgataacttc tgccaccgat acattagctg tttgaagggg
      541 aaaatgccca tcgacctcgt cattgatgaa agagacggca gctccaagtc agatcatgaa
      601 gaactttcag gctcctccac aaatctcgct gaccataacc cttcttcttg gcgagaccac
      661 gatgatgcaa cctcaaccca ctcagcaggc accccagggc cctccagtgg gggccatgct
      721 tcccagagcg gagacaacag cagtgagcaa ggggatggtt tagacaacag tgtagcttca
      781 cctggtacag gtgacgatga tgatccggat aaggacaaaa aacgccagaa gaaaagaggc
      841 attttcccca aagtagcaac aaatatcatg agagcatggc tcttccagca tctcacacat
      901 ccgtaccctt ccgaagagca gaagaaacag ttagcgcaag acacaggact tacaattctc
      961 caagtaaaca actggtttat taatgccaga agaagaatag tacagcccat gattgaccag
     1021 tcaaatcgag cagtgagcca aggagcagca tatagtccag agggtcagcc catggggagc
     1081 tttgtgttgg atggtcagca acacatgggg atccggcctg caggacctat gagtggaatg
     1141 ggcatgaata tgggcatgga tgggcaatgg cactacatgt acccaacttt cttgtacaaa
     1201 gtggttgata tcggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa
     1261 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1321 gacgaacaca cccaatcgga cacgtgcag
//
