LOCUS       sequence-286449-         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3302
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(782..806)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             814..855
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga cgtgtgttga acaagacaag
       61 ctgggtcaag catttgaaga tgcttttgag gttctgaggc aacattcaac tggagatctt
      121 cagtactcgc cagattacag aaattacctg gctttaatca accatcgtcc tcatgtcaaa
      181 ggaaattcca gctgctatgg agtgttgcct acagaggagc ctgtctataa ttggagaacg
      241 gtaattaaca gtgctgcgga cttctatttt gaaggaaata ttcatcaatc tctgcagaac
      301 ataactgaaa accagctggt acaacccact cttctccagc aaaagggggg aaaaggcagg
      361 aagaagctcc gactgtttga ataccttcac gaatccctgt ataatccgga gatggcatct
      421 tgtattcagt gggtagataa aaccaaaggc atctttcagt ttgtatcaaa aaacaaagaa
      481 aaacttgccg agctttgggg gaaaagaaaa ggcaacagga agaccatgac ttaccagaaa
      541 atggccaggg cactcagaaa ttacggaaga agtggggaaa ttaccaaaat ccggaggaag
      601 ctgacttacc agttcagtga ggccattctc caaagactct ctccatccta tttcctgggg
      661 aaagagatct tctattcaca gtgtgttcaa cctgatcaag aatatctcag tttaaataac
      721 tggaatgcaa attataatta tacatatgcc aattaccatg agctaaatca ccatgattgc
      781 tacccaactt tcttgtacaa agtggttgat atcggtaagc ctatccctaa ccctctcctc
      841 ggtctcgatt ctacgtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
      901 ctttatatat cttgtggaaa ggacgaccgt atctttatag aatcctcgga
//
