LOCUS       sequence-286458-        1994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3305
FEATURES             Location/Qualifiers
     source          1..1994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             886..1821
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             934..1824
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     protein_bind    complement(1826..1850)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1858..1899
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgaccatgac cctccacacc
       61 aaagcatctg ggatggccct actgcatcag atccaaggga acgagctgga gcccctgaac
      121 cgtccgcagc tcaagatccc cctggagcgg cccctgggcg aggtgtacct ggacagcagc
      181 aagcccgccg tgtacaacta ccccgagggc gccgcctacg agttcaacgc cgcggccgcc
      241 gccaacgcgc aggtctacgg tcagaccggc ctcccctacg gccccgggtc tgaggctgcg
      301 gcgttcggct ccaacggcct ggggggtttc cccccactca acagcgtgtc tccgagcccg
      361 ctgatgctac tgcacccgcc gccgcagctg tcgcctttcc tgcagcccca cggccagcag
      421 gtgccctact acctggagaa cgagcccagc ggctacacgg tgcgcgaggc cggcccgccg
      481 gcattctaca ggccaaattc agataatcga cgccagggtg gcagagaaag attggccagt
      541 accaatgaca agggaagtat ggctatggaa tctgccaagg agactcgcta ctgtgcagtg
      601 tgcaatgact atgcttcagg ctaccattat ggagtctggt cctgtgaggg ctgcaaggcc
      661 ttcttcaaga gaagtattca aggacataac gactatatgt gtccagccac caaccagtgc
      721 accattgata aaaacaggag gaagagctgc caggcctgcc ggctccgcaa atgctacgaa
      781 gtgggaatga tgaaaggtgg gatacgaaaa gaccgaagag gagggagaat gttgaaacac
      841 aagcgccaga gagatgatgg ggagggcagg ggtgaagtgg ggtctgctgg agacatgaga
      901 gctgccaacc tttggccaag cccgctcatg atcaaacgct ctaagaagaa cagcctggcc
      961 ttgtccctga cggccgacca gatggtcagt gccttgttgg atgctgagcc ccccatactc
     1021 tattccgagt atgatcctac cagacccttc agtgaagctt cgatgatggg cttactgacc
     1081 aacctggcag acagggagct ggttcacatg atcaactggg cgaagagggt gccaggcttt
     1141 gtggatttga ccctccatga tcaggtccac cttctagaat gtgcctggct agagatcctg
     1201 atgattggtc tcgtctggcg ctccatggag cacccaggga agctactgtt tgctcctaac
     1261 ttgctcttgg acaggaacca gggaaaatgt gtagagggca tggtggagat cttcgacatg
     1321 ctgctggcta catcatctcg gttccgcatg atgaatctgc agggagagga gtttgtgtgc
     1381 ctcaaatcta ttattttgct taattctgga gtgtacacat ttctgtccag caccctgaag
     1441 tctctggaag agaaggacca tatccaccga gtcctggaca agatcacaga cactttgatc
     1501 cacctgatgg ccaaggcagg cctgaccctg cagcagcagc accagcggct ggcccagctc
     1561 ctcctcatcc tctcccacat caggcacatg agtaacaaag gcatggagca tctgtacagc
     1621 atgaagtgca agaacgtggt gcccctctat gacctgctgc tggagatgct ggacgcccac
     1681 cgcctacatg cgcccactag ccgtggaggg gcatccgtgg aggagacgga ccaaagccac
     1741 ttggccactg cgggctctac ttcatcgcat tccttgcaaa agtattacat cacgggggag
     1801 gcagagggtt tccctgccac ggtcttgcca actttcttgt acaaagtggt tgatatcggt
     1861 aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgaac tagtccgtaa
     1921 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga ctttagaggt
     1981 aacgcatgaa catc
//
