LOCUS       sequence-286461-         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3306
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(677..701)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             709..750
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg tgcagtcctg ctccgcctac
       61 ggctgcaaga accgctacga caaggacaag cccgtttctt tccacaagtt tcctcttact
      121 cgacccagtc tttgtaaaga atgggaggca gctgtcagaa gaaaaaactt taaacccacc
      181 aagtatagca gtatttgttc agagcacttt actccagact gctttaagag agagtgcaac
      241 aacaagttac tgaaagagaa tgctgtgccc acaatatttc tttgtactga gccacatgac
      301 aagaaagaag atcttctgga gccacaggaa cagcttcccc cacctccttt accgcctcct
      361 gtttcccagg ttgatgctgc tattggatta ctaatgccgc ctcttcagac ccctgttaat
      421 ctctcagttt tctgtgacca caactatact gtggaggata caatgcacca gcggaaaagg
      481 attcatcagc tagaacagca agttgaaaaa ctcagaaaga agctcaagac cgcacagcag
      541 cgatgcagaa ggcaagaacg gcagcttgaa aaattaaagg aggttgttca cttccagaaa
      601 gagaaagacg acgtatcaga aagaggttat gtgattctac caaatgacta ctttgaaata
      661 gttgaagtac cagcataccc aactttcttg tacaaagtgg ttgatatcgg taagcctatc
      721 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
      781 atttcgattt cttggcttta tatatcttgt ggaaaggacg atagggcgta gacgaccctt
      841 aataa
//
