LOCUS       sequence-286464-        1634 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3307
FEATURES             Location/Qualifiers
     source          1..1634
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1466..1490)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1498..1539
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg acgcagtggc ctttgaggat
       61 gtggctgtga acttcaccca ggaggaatgg gctttgctgg gtccatcaca gaagaattta
      121 tacagatatg tgatgcaaga aaccatcagg aacctggact gtataagaat gatatgggaa
      181 gaacagaata ctgaagatca gtacaaaaat cctaggagaa atctaagatg tcatatggta
      241 gagagattca gtgaaagtaa agacagtagt cagtgtggag aaacatttag cctcattcga
      301 gatagtattg tgaacaacag catttgtcct ggagaagatc catgtcaaag cgctgagtgt
      361 gaagaagtca taatgggtca tttatccctt aatagccaca tcagagttga ttctggacac
      421 aaaccacatg agtatcagga atatggagag aagccacata cacataaaca acgtgggaaa
      481 gccttcagtt accatcactc ctttcagtcg cgtggaaggc ctcacactgg aaagaaacgc
      541 tatgagtgta aggaatgtgg aaaaaccttc agttctcgta gaaaccttcg aagacacatg
      601 gtagtgcaag gtggaaatag accttataaa tgtaagttgt gtgggaaagc ttttttttgg
      661 cccagtttat tacgtatgca tgaaagaact cacactggag agaaaccgta tgaatgtaag
      721 cagtgttcta aagcctttcc tttttacagt tcctatcgaa gacatgagag aatgcacact
      781 ggggagaaac cgtatgaatg taagcagtgt tctaaagcct tgcctgattc cagttcctat
      841 ataagacatg aaagaactca cactggagag aaaccatata catgtaaaca gtgtgggaaa
      901 gccttcagtg tttccagttc ccttcgaaga catgaaacca ctcacagtgc agagaaaccc
      961 tatgagtgta agcaatgcgg gaaaacattt catcatcttg gaagctttca gatacacatg
     1021 aaaaggcaca ctggagatcg acctcataaa tgtaagatat gtgggaaagg ctttgatcgt
     1081 cccagtttag ttcgatatca tgaacgaatt cacactggag agaaacccta tgaatgcaag
     1141 cagtgtggga aaacgttatc tcatagctca agctttcgaa gacacatgat aatgcacact
     1201 ggaggtggac ctcataaatg caagatatgt gggaaagctt ttgtttatcc tagtgtatgt
     1261 caaagacacg aaaagtctca cagtggagag aaaccctatg aatgcaagca gtgtgggaaa
     1321 gcgttatctc atagctcaag ctttcgaaga catatggtaa tgcatacggg agatgggccg
     1381 aataaatgca aggtatgtgg gaaagccttt gtttatccca gtgtatgtca aagacatgaa
     1441 aagactcact ggagagaaac aatatgccca actttcttgt acaaagtggt tgatatcggt
     1501 aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgaac tagtccgtaa
     1561 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga acgcttaggc
     1621 taatgtcctt gtgc
//
