LOCUS       sequence-286467-        1640 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3308
FEATURES             Location/Qualifiers
     source          1..1640
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1472..1496)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1504..1545
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cctgcctcaa cccggtgcct
       61 aaactctaca gatctgtaat tgaagatgta attgaaggag ttcggaatct atttgctgaa
      121 gaaggtatag aggaacaagt tttaaaagac ttgaagcagc tctgggaaac caaggttttg
      181 cagtctaaag caacagaaga cttcttcaga aatagcatcc aatcacctct gtttactctt
      241 cagttgccgc acagcttgca ccaaacattg caatcgtcaa cagcatcatt agttattcct
      301 gctggtagaa ctcttccaag ttttaccaca gcagaactgg gcacttcaaa ctccagtgca
      361 aactttactt ttcctggtta tcccattcat gtaccagcag gtgtgacact acagactgta
      421 tctggtcacc tttataaagt caatgtacca attatggtga cagagacttc tggaagagca
      481 ggtattcttc agcatccaat tcagcaagta tttcaacagc ttggccagcc ttcagtaata
      541 caaactagtg ttccacaatt gaatccatgg tctcttcaag caactactga aaaatcacag
      601 agaattgaaa ccgtgctaca gcaacccgca attctacctt ctgggccagt agataggaaa
      661 cacttagaaa atgccaccag tgatatactt gtatctcctg gaaatgagca taaaatcgtg
      721 cctgaagctt tgttgtgtca tcaggaaagt tctcactata tcagtcttcc aggtgttgta
      781 ttttctccac aggtctctca aacaaattct aatgtggagt cagtgctcag tggttcagct
      841 agcatggctc aaaatctgca tgatgagtcc ctctccacaa gccctcatgg ggctctccac
      901 cagcacgtga ctgatattca gcttcatatt cttaaaaata ggatgtatgg atgtgattct
      961 gtaaagcaac caagaaatat agaggaaccc agcaacatac ctgtatcaga gaaggattct
     1021 aattctcagg tggatttaag cattcgggtt actgatgatg atattggtga aataattcaa
     1081 gtagatggaa gcggtgatac atcttccaat gaagaaatag gaagtacaag agatgcagat
     1141 gagaatgaat ttctagggaa tattgacggg ggagatctga aggtacctga agaagaagct
     1201 gacagtattt caaatgagga ttcagccaca aacagtagtg ataatgaaga ccctcaagta
     1261 aacattgtag aagaggaccc tttaaattct ggagatgatg ttagtgaaca ggatgtgcca
     1321 gacctgtttg acacggataa tgttattgtc tgtcagtatg ataagattca tcgaagcaag
     1381 aacaaatgga aattctattt gaaagatggt gttatgtgtt ttggagggag agactatgta
     1441 tttgcaaaag ccattggtga tgcagagtgg tacccaactt tcttgtacaa agtggttgat
     1501 atcggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgaactagt
     1561 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgagcat
     1621 attcagagcc cgacatatgc
//
