LOCUS       sequence-286470-        1952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3309
FEATURES             Location/Qualifiers
     source          1..1952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1784..1808)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1816..1857
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg aggcgctgag tcgagctggg
       61 caggagatga gcctagcggc cctgaagcaa cacgacccct atatcaccag catcgcagac
      121 ctcacgggcc aggtcgctct gtacaccttc tgccccaagg ccaaccagtg ggagaagact
      181 gatatagaag ggaccttatt cgtatatcga aggtcagctt ccccttacca tggttttacc
      241 attgtgaatc gactaaatat gcacaatcta gttgaaccag tgaataaaga tttggaattt
      301 cagctccatg aaccatttct tctgtataga aatgcaagct tgtcgatata tagtatctgg
      361 ttttatgaca agaatgactg tcaccgcata gcaaaactca tggctgatgt ggtagaagag
      421 gagacacggc gatcccagca agctgctcgg gacaaacaga gtcccagcca ggccaatggc
      481 tgcagcgacc acaggcccat cgacatcctg gagatgctga gcagagccaa ggatgagtat
      541 gagaggaatc agatgggtga ctcaaatatc tccagccctg ggttacagcc aagcactcag
      601 ctctccaatc tgggaagcac cgagactcta gaagaaatgc cctccgggtc acaggataag
      661 tctgctccat ctggacacaa gcatctgacg gtagaagagt tatttggaac ctctttgcca
      721 aaggaacaac cagcagttgt gggtctggat tcagaagaaa tggagaggtt gccaggagat
      781 gcctcccaga aagagcccaa ttcattccta ccatttccct ttgagcagtt aggaggagcc
      841 cctcaatcag aaaccctggg tgtcccttct gctgcccacc attcagtcca gcctgaaatc
      901 accaccccgg tgctaatcac tccagcctcc atcacacagt ccaatgaaaa gcatgctcca
      961 acctacacaa tcccgttgag ccctgttctc agtcccactc tgccagctga agctcctact
     1021 gcacaggttc cccccagctt acctcgaaac agcaccatga tgcaggcagt gaagaccacg
     1081 cctagacaga ggtctccact cctgaaccag ccagtccctg agctaagcca tgccagtctg
     1141 attgccaacc agagcccctt cagggcccca ttgaacgtga cgaacacagc tggcacatcc
     1201 ctcccaagcg ttgatcttct ccagaaactc aggttgaccc cacagcatga ccaaatacag
     1261 acacaaccac ttgggaaagg tgcaatggta gccagctttt ctccggcagc tggtcagcta
     1321 gccacacctg agagcttcat agagcctccc tctaagacag cagcagcaag agtggcggcc
     1381 tcagcctccc tgagcaacat ggtgcttgct ccccttcagt ctatgcagca gaaccaggat
     1441 cctgaagtat ttgtgcagcc taaggtgtta tccagtgcca tcccggttgc aggcgcccca
     1501 ctggttactg caacgaccac tgcagtgtct tcagtcctgc tggccccaag tgttttccag
     1561 cagacagtta caagatcttc ggaccttgag aggaaagcca gctccccttc tcctctaact
     1621 attggaacgc cagaaagtca gagaaagcct tccattattc tcagcaagtc tcagctccag
     1681 gatacattaa tacatctaat aaagaatgat tccagcttcc tcagtacact tcatgaagtc
     1741 tacttgcagg ttctgaccaa gaacaaagac aaccacaacc tatgcccaac tttcttgtac
     1801 aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag
     1861 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1921 aaggacgaat tattaccgat ccattactga cg
//
