LOCUS       sequence-286473-        1457 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3310
FEATURES             Location/Qualifiers
     source          1..1457
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1289..1313)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1321..1362
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc tggagaacta catgaacctg
       61 gcctctgtgg aatgggaaat acaacctaga accaaacggt catcacttca gcagggtttt
      121 ttgaagaatc aaatattcag tgggatacaa atgacaagag gctacagtgg atggaaactc
      181 tgtgactgta agaattgtgg agaggtcttc agggaacagt tttgccttaa gacacacatg
      241 agagttcaga atggagggaa tacttctgag ggtaattgtt atggaaaaga caccctcagt
      301 gtgcacaagg aagcctctac tggacaggaa ctttccaaat ttaatccatg tggaaaagtc
      361 tttactctaa ctccaggtct tgctgtacat cttgaagttc tcaatgcaag acaaccctac
      421 aaatgtaagg aatgtggaaa aggctttaag tattttgcaa gccttgataa tcatatggga
      481 atccacactg atgagaaact ctgtgaattt caggaatatg ggagagctgt cacagcttct
      541 tcacacctaa agcagtgtgt agcagttcat acaggaaaga aatccaaaaa gactaagaaa
      601 tgtgggaaat ccttcactaa tttttctcaa ctttatgcac ctgtgaaaac tcataaagga
      661 gagaagtcct ttgaatgtaa agaatgtgga agatccttta gaaattcctc atgccttaat
      721 gatcacattc aaattcacac tggaataaaa ccacacaagt gtacttactg tgggaaagcc
      781 ttcactagat caactcaact tactgaacat gtaagaactc acactggaat aaaaccctat
      841 gaatgtaagg aatgtggcca agcctttgct cagtactcgg gcctttctat acacatacga
      901 agtcacagtg gaaagaaacc ctatcagtgt aaggaatgtg ggaaagcctt cactacatcc
      961 acaagtctta ttcagcatac aagaattcac acaggagaga agccttatga atgtgttgaa
     1021 tgtgggaaga ccttcattac ttcttcccgt cgtagtaaac atttgaaaac tcatagtgga
     1081 gaaaagccct ttgtatgcaa gatatgtggg aaagcatttc tatattcctc acgccttaat
     1141 gttcacctgc gaactcatac cggagagaaa cccttcgtat gtaaagaatg tgggaaagca
     1201 tttgctgttt cctcacgcct aagtagacat gaaagaattc acactgggga gaaaccctat
     1261 gaatgcaagg atatgagtgt taccatttac ccaactttct tgtacaaagt ggttgatatc
     1321 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtccg
     1381 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgacaagaat
     1441 agaaaatagc taacttc
//
