LOCUS       sequence-286476-        1319 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3311
FEATURES             Location/Qualifiers
     source          1..1319
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1151..1175)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1183..1224
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg agctggaatt ggatgctggt
       61 gaccaagacc tgctggcctt cctgctagag gaaagtggag atttggggac ggcacccgat
      121 gaggccgtga gggccccact ggactgggcg ctgccgcttt ctgaggtacc gagcgactgg
      181 gaagtagatg atttgctgtg ctccctgctg agtcccccag cgtcgttgaa cattctcagc
      241 tcctccaacc cctgccttgt ccaccatgac cacacctact ccctcccacg ggaaactgtc
      301 tctatggatc tagagagtga gagctgtaga aaagagggga cccagatgac tccacagcat
      361 atggaggagc tggcagagca ggagattgct aggctagtac tgacagatga ggagaagagt
      421 ctattggaga aggaggggct tattctgcct gagacacttc ctctcactaa gacagaggaa
      481 caaattctga aacgtgtgcg gaggaagatt cgaaataaaa gatctgctca agagagccgc
      541 aggaaaaaga aggtgtatgt tgggggttta gagagcaggg tcttgaaata cacagcccag
      601 aatatggagc ttcagaacaa agtacagctt ctggaggaac agaatttgtc ccttctagat
      661 caactgagga aactccaggc catggtgatt gagatatcaa acaaaaccag cagcagcagc
      721 acctgcatct tggtcctact agtctccttc tgcctcctcc ttgtacctgc tatgtactcc
      781 tctgacacaa gggggagcct gccagctgag catggagtgt tgtcccgcca gcttcgtgcc
      841 ctccccagtg aggaccctta ccagctggag ctgcctgccc tgcagtcaga agtgccgaaa
      901 gacagcacac accagtggtt ggacggctca gactgtgtac tccaggcccc tggcaacact
      961 tcctgcctgc tgcattacat gcctcaggct cccagtgcag agcctcccct ggagtggcca
     1021 ttccctgacc tcttctcaga gcctctctgc cgaggtccca tcctccccct gcaggcaaat
     1081 ctcacaagga agggaggatg gcttcctact ggtagcccct ctgtcatttt gcaggacaga
     1141 tactcaggct acccaacttt cttgtacaaa gtggttgata tcggtaagcc tatccctaac
     1201 cctctcctcg gtctcgattc tacgtagtaa tgaactagtc cgtaacttga aagtatttcg
     1261 atttcttggc tttatatatc ttgtggaaag gacgataccg acccatttgt ccggcccga
//
