LOCUS       sequence-286479-        1421 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3312
FEATURES             Location/Qualifiers
     source          1..1421
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1253..1277)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1285..1326
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg acagtgctat caccctgtgg
       61 cagttccttc ttcagctcct gcagaagcct cagaacaagc acatgatctg ttggacctct
      121 aatgatgggc agtttaagct tttgcaggca gaagaggtgg ctcgtctctg ggggattcgc
      181 aagaacaagc ctaacatgaa ttatgacaaa ctcagccgag ccctcagata ctattatgta
      241 aagaatatca tcaaaaaagt gaatggtcag aagtttgtgt acaagtttgt ctcttatcca
      301 gagattttga acatggatcc aatgacagtg ggcaggattg agggtgactg tgaaagttta
      361 aacttcagtg aagtcagcag cagttccaaa gatgtggaga atggagggaa agataaacca
      421 cctcagcctg gtgccaagac ctctagccgc aatgactaca tacactctgg cttatattct
      481 tcatttactc tcaactcttt gaactcctcc aatgtaaagc ttttcaaatt gataaagact
      541 gagaatccag ccgagaaact ggcagagaaa aaatctcctc aggagcccac accatctgtc
      601 atcaaatttg tcacgacacc ttccaaaaag ccaccggttg aacctgttgc tgccaccatt
      661 tcaattggcc caagtatttc tccatcttca gaagaaacta tccaagcttt ggagacattg
      721 gtttccccaa aactgccttc cctggaagcc ccaacctctg cctctaacgt aatgactgct
      781 tttgccacca caccacccat ttcgtccata ccccctttgc aggaacctcc cagaacacct
      841 tcaccaccac tgagttctca cccagacatc gacacagaca ttgattcagt ggcttctcag
      901 ccaatggaac ttccagagaa tttgtcactg gagcctaaag accaggattc agtcttgcta
      961 gaaaaggaca aagtaaataa ttcatcaaga tccaagaaac ccaaagggtt agaactggca
     1021 cccacccttg tgatcacgag cagtgatcca agcccactgg gaatactgag cccatctctc
     1081 cctacagctt ctcttacacc agcatttttt tcacaggtag cttgctcgct ctttatggtg
     1141 tcaccattgc tttcatttat ttgccctttt aagcaaatcc agaatttata cactcaagtt
     1201 tgctttctgt tacttaggtt tgtcttagaa aggttatgtg tgactgtcat gtgcccaact
     1261 ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct cggtctcgat
     1321 tctacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1381 tcttgtggaa aggacgactt ggacctcctt ggtccaacct t
//
