LOCUS       sequence-286482-        1511 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3313
FEATURES             Location/Qualifiers
     source          1..1511
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1343..1367)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1375..1416
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg acactgccag ccatagcctt
       61 gttcttctcc agcagctgaa catgcagcga gaatttggtt ttctgtgtga ttgcacagtt
      121 gcaattggag atgtttactt caaagcccac agagcagtgc ttgctgcttt ttctaactat
      181 ttcaagatga tatttattca ccaaacaagt gaatgcataa aaatacaacc aactgacatc
      241 caacctgaca tattcagcta tttgttgcac attatgtaca cggggaaagg gccaaaacag
      301 attgtggatc atagtcgttt ggaggaaggg attcgatttc ttcacgccga ctacctttct
      361 cacattgcaa ctgaaatgaa tcaagtgttc tcaccagaga ctgtgcagtc ctcaaattta
      421 tatggcattc agatctcaac aacccaaaaa acagttgtca aacaaggact ggaggtcaaa
      481 gaagctcctt ccagtaacag tggaaacaga gctgctgtcc agggtgacca cccccagttg
      541 cagttgtctc ttgctattgg tctggatgat ggcactgcag accagcagag ggcctgtcct
      601 gccacccagg ccctggagga gcaccagaag cccccagttt ccatcaagca ggagagatgt
      661 gacccagaat ctgtgatctc ccagagccac ccctcaccct catcagaggt gacaggcccc
      721 acttttactg aaaacagtgt caaaatacac ttatgccatt actgtgggga acgttttgat
      781 tcccgtagta acctaaggca acatctccat acacatgtgt ctggatccct gccattcggt
      841 gtccctgctt ccattctgga aagtaatgac cttggtgaag tgcatcccct taatgaaaac
      901 agcgaggccc ttgaatgccg caggctcagc tccttcattg ttaaggagaa tgaacagcag
      961 ccagaccaca ccaaccgggg taccacagag cctttgcaga tcagtcaagt atctttgatc
     1021 tccaaagaca cagagccagt agaattaaac tgtaattttt ctttttcaag gaaaagaaaa
     1081 atgagctgta ccatctgtgg tcataaattc cctcgaaaga gccaattgtt ggaacacatg
     1141 tatacacaca aaggtaaatc ttacagatat aaccgatgcc aaaggtttgg taatgcattg
     1201 gcccagagat ttcagccata ctgtgacagc tggtctgatg tctccctgaa aagttctcgc
     1261 ttgtcacaag aacacttaga cttgccttgt gccttagagt cagagctcac acaagaaaat
     1321 gtggatacta tcctagttga gtacccaact ttcttgtaca aagtggttga tatcggtaag
     1381 cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag tccgtaactt
     1441 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgacag cgcagagcac
     1501 taagcaggtt g
//
