LOCUS       sequence-286488-        2195 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3315
FEATURES             Location/Qualifiers
     source          1..2195
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2027..2051)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2059..2100
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg ctattaccct acagcccagt
       61 gacctgatct ttgagttcgc aagcaacggg atggatgatg atatccacca gctggaagac
      121 ccctctgtgt tcccagctgt gatcgtggag caggtaccct accctgattt actgcatctg
      181 tactcgggac tggagttgga cgacgttcac aatggcatca taacagacgg gaccttgtgc
      241 atgacgcagg atcagatcct ggaaggcagt tttttgctga cagatgacaa tgaggccacc
      301 tcgcacacca tgtcaaccgc ggaagtctta ctcaatatgg agtctcccag cgatatcctg
      361 gatgagaagc agatcttcag tacctccgaa atgcttccag actcggaccc tgcaccagct
      421 gtcactctgc ccaactacct gtttcctgcc tctgagcccg atgccctgaa cagggcgggt
      481 gacactagtg accaggaggg gcattctctg gaggagaagg cctccagaga ggaaagtgcc
      541 aagaagactg ggaaatcaaa gaagagaatc cggaagacca agggcaaccg aagtacctca
      601 cctgtcactg accccagcat ccccattagg aagaaatcaa aggatggcaa aggcagcacc
      661 atctatctgt gggagttcct cctggctctt ctgcaagaca gaaacacctg tcccaagtac
      721 atcaagtgga cccagcgaga gaaaggcatc ttcaaactgg tggactccaa agctgtgtcc
      781 aagctgtggg ggaagcagaa aaacaagcct gacatgaact atgagacaat ggggcgggca
      841 ctaagatact actaccaaag aggcatactg gccaaagtgg aagggcagag gctggtgtac
      901 cagtttaagg agatgcccaa ggacctggtg gtcattgaag atgaggatga gagcagcgaa
      961 gccacagcag ccccacctca ggcctccacg gcctctgtgg cctctgccag taccacccgg
     1021 cgaaccagct ccagggtctc atccagatct gccccccagg gcaagggcag ctcttcttgg
     1081 gagaagccaa aaattcagca tgtcggtctc cagccatctg cgagtctgga attgggaccg
     1141 tcgctagacg aggagatccc cactacctcc accatgctcg tctctccagc agagggccag
     1201 gtcaagctca ccaaagctgt gagtgcatct tcagtgccca gcaacatcca cctaggagtg
     1261 gcccccgtgg ggtcgggctc ggccctgacc ctgcagacga tcccactgac cacggtgctg
     1321 accaatgggc ctcctgccag tactactgct cccactcagc tcgttctcca gagtgttcca
     1381 gcggcctcta ctttcaagga caccttcact ttgcaggcct ctttccccct gaacgccagt
     1441 ttccaagaca gccaggtggc agccccaggg gctccactga ttctcagtgg cctcccccaa
     1501 cttctggctg gggccaaccg tccgaccaac ccggcgccac ccacggtcac aggggctgga
     1561 ccagcagggc ccagctctca gccccctggg actgtcattg ctgccttcat caggacttct
     1621 ggcactacag cagcccctag ggtcaaggag gggccactga ggtcctcctc ctatgttcag
     1681 ggtatggtga cgggggcccc catggagggg ctgctggttc ctgaagagac cctgagggag
     1741 ctcctgagag atcaggctca tcttcagcca cttccaaccc aggtggtttc caggggttcc
     1801 cacaatccga gccttctggg caaccagact ttgtctcctc ccagccgccc cactgttggg
     1861 ctgaccccag tggctgaact tgagctctcc tcaggctcag ggtccctgct gatggctgag
     1921 cctagtgtga ccacatctgg gagccttctg acaagatccc ccaccccagc ccctttctcc
     1981 ccattcaacc ctacttccct cattaagatg gagccccatg acatataccc aactttcttg
     2041 tacaaagtgg ttgatatcgg taagcctatc cctaaccctc tcctcggtct cgattctacg
     2101 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     2161 ggaaaggacg ataacaaaac tgaggtaaac tttac
//
