LOCUS       sequence-286491-         638 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3316
FEATURES             Location/Qualifiers
     source          1..638
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(470..494)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             502..543
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cggatcggct cacgcagctt
       61 caggacgctg tgaattcgct tgcagatcag ttttgtaatg ccattggagt attgcagcaa
      121 tgtggtcctc ctgcctcttt caataatatt cagacagcaa ttaacaaaga ccagccagct
      181 aaccctacag aagagtatgc ccagcttttt gcagcactga ttgcacgaac agcaaaagac
      241 attgatgttt tgatagattc cttacccagt gaagaatcta cagctgcttt acaggctgct
      301 agcttgtata agctagaaga agaaaaccat gaagctgcta catgtctgga ggatgttgtt
      361 tatcgaggag acatgcttct ggagaagata caaagcgcac ttgctgatat tgcacagtca
      421 cagctgaaga caagaagtgg tacccatagc cagtctcttc cagactcata cccaactttc
      481 ttgtacaaag tggttgatat cggtaagcct atccctaacc ctctcctcgg tctcgattct
      541 acgtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
      601 tgtggaaagg acgagtttta ccacttatga aaatccga
//
