LOCUS       sequence-286494-        1097 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3317
FEATURES             Location/Qualifiers
     source          1..1097
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(929..953)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             961..1002
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgtatttttc ccaggaagaa
       61 tgggagttat tggatcccac tcagaaggcc ctctacaatg atgtaatgca ggaaaactat
      121 gagactgtca tctctctagc attgtttgtg ctccccaaac ctaaagtgat ctcctgtcta
      181 gagcaagggg aagagccatg ggttcaagta tccccggagt ttaaggatag tgccggaaaa
      241 tctcctacag ggttaaagct caaaaacgac actgaaaatc atcagcctgt gtctctttct
      301 gacttagaaa tacaagcatc agcaggcgtc atatcaaaaa aggccaaagt aaaagttccc
      361 cagaaaacag caggcaaaga aaatcatttt gatatgcaca gagtgggaaa atggcaccaa
      421 gattttccag tgaagaaaag aaagaaactt tcaacctgga aacaagagct gctcaaactt
      481 atggatcgtc acaagaaaga ttgtgcaaga gagaagcctt ttaaatgtca ggaatgtggg
      541 aaaaccttca gagttagctc tgaccttatt aagcaccaaa gaattcacac tgaagagaaa
      601 ccctataaat gtcaacagtg tgataagagg tttagatgga gttcagatct taataagcac
      661 ttaacaacac accaaggaat aaaaccatat aaatgttcat ggtgtgggaa aagcttcagt
      721 caaaatacaa atttacatac acaccaaaga actcatacag gagaaaagcc cttcacatgt
      781 catgaatgtg gaaaaaaatt cagtcagaac tcccacctta ttaaacaccg gagaacccac
      841 acaggtgagc agccatatac ttgtagcata tgcaggagaa acttcagcag gcggtcaagc
      901 cttcttagac accagaaact ccacctgttg ccaactttct tgtacaaagt ggttgatatc
      961 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtccg
     1021 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaatacca
     1081 tgaccaacaa taagccc
//
